Team:Waterloo/Safety

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<h3>SAFETY</h3>
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<br><h4>Laboratory Safety</h4><p>
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The Ribozyme Project is not expected to raise any research, public or environmental safety concerns other than those normally associated with Biosafety Level 2 organisms, such as Escherichia coli (DH5-alpha), which is classified as very low to moderate. The use of this project is primarily reserved for research and laboratory use, therefore, should not purposefully be exposed to the public or environment except after further testing in its specific applications (such as with particular fusion proteins). Furthermore, the basis of our project is to establish a self-excising sequence (ribozymes), which should limit the expression of any intervening sequences to the RNA level. If the intervening sequence were something of environmental or public relevance (such as antibiotic resistance), the experimental design indicates that the sequence will be removed and, thus, not expressed. This is a relevant contribution of the design in limiting expression to the RNA level, which eases environmental hazard concern upon the accidental release of a GMO containing this biobrick. Therefore, the new biobrick parts submitted should not raise any safety issues.
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</p>
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<br/><p><b>The necessary facility, equipment and handling procedures associated with Level 2 Biosafety concerns were met:</b>
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<br/>
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<br/>1.Pipetting aids
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<br/>2.Biosafety cabinets where applicable
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<br/>3.Laboratory separated from other activities
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<br/>4.Biohazard sign
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<br/>5.Proper safety and disposal equipment, including autoclave
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<br/>6.Personal protective equipment, worn only in the laboratory
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<br/>7.Screw-capped tubes and bottles
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<br/>8.Plastic disposable pasteur pipettes, when necessary
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</p>
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<br/><br/>
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<p>
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<b>All precautions with respect to recombinant DNA were observed:</b>
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<br/>1.All waste was autoclaved before being thrown away.
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<br/>2.Researchers practiced aseptic technique and personal hygiene and safety precautions
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<br/>3.Procedures likely to generate aerosols are performed in a biosafety cabinet
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<br/>4.Bench surfaces were disinfected with ethanol.
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<br/>4.Potentially contaminated waste is separated from general waste
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Use this page to answer the questions on the  [[Safety | safety page]].
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  [[Safety | safety page]].

Revision as of 05:21, 7 September 2012


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SAFETY


Laboratory Safety

The Ribozyme Project is not expected to raise any research, public or environmental safety concerns other than those normally associated with Biosafety Level 2 organisms, such as Escherichia coli (DH5-alpha), which is classified as very low to moderate. The use of this project is primarily reserved for research and laboratory use, therefore, should not purposefully be exposed to the public or environment except after further testing in its specific applications (such as with particular fusion proteins). Furthermore, the basis of our project is to establish a self-excising sequence (ribozymes), which should limit the expression of any intervening sequences to the RNA level. If the intervening sequence were something of environmental or public relevance (such as antibiotic resistance), the experimental design indicates that the sequence will be removed and, thus, not expressed. This is a relevant contribution of the design in limiting expression to the RNA level, which eases environmental hazard concern upon the accidental release of a GMO containing this biobrick. Therefore, the new biobrick parts submitted should not raise any safety issues.


The necessary facility, equipment and handling procedures associated with Level 2 Biosafety concerns were met:

1.Pipetting aids
2.Biosafety cabinets where applicable
3.Laboratory separated from other activities
4.Biohazard sign
5.Proper safety and disposal equipment, including autoclave
6.Personal protective equipment, worn only in the laboratory
7.Screw-capped tubes and bottles
8.Plastic disposable pasteur pipettes, when necessary



All precautions with respect to recombinant DNA were observed:
1.All waste was autoclaved before being thrown away.
2.Researchers practiced aseptic technique and personal hygiene and safety precautions
3.Procedures likely to generate aerosols are performed in a biosafety cabinet
4.Bench surfaces were disinfected with ethanol.
4.Potentially contaminated waste is separated from general waste safety page.

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