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Team:Cambridge/Protocols/SporeMedia
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(Created page with "=CDSM= ==Sporulation Induction Media== *40mM morpholinopropanesulfonic acid (MOPS) *4.0mM KH<sub>2</sub>PO<sub>4</sub> *9.5mM (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub> *5.0mM ...")
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Revision as of 16:20, 5 September 2012
CDSM
Sporulation Induction Media
- 40mM morpholinopropanesulfonic acid (MOPS)
- 4.0mM KH2PO4
- 9.5mM (NH4)2SO4
- 5.0mM L-lactic acid
- 8.0mM L-Glutamic acid
- 20mM - D-Glucose
- 1xMT mix
50xMT mix (trace metal mix)
Note: Add FeCl3 last to avoid precipitation
For 1L:
- 2ml 1M HCl
- 40.6g Mg Cl2
- 10.29g CaCl2
- 0.99g MnCl2
- 13.6g ZnCl2
- 67.5mg Thiamine-HCL
- 135mg FeCl3
