"
Team:USTC-China/Methods
From 2012.igem.org
| Line 102: | Line 102: | ||
<a href="http://www.koodoz.com.au/portfolio/web-design/"><img src="http://www.koodoz.com.au/wp-content/themes/koodoz/style/project-images/image.php?i=W-HOC0001-1.jpg&w=200" alt="project image" style="float:left;"></a><br><small align="center">Some description of the picture</small> | <a href="http://www.koodoz.com.au/portfolio/web-design/"><img src="http://www.koodoz.com.au/wp-content/themes/koodoz/style/project-images/image.php?i=W-HOC0001-1.jpg&w=200" alt="project image" style="float:left;"></a><br><small align="center">Some description of the picture</small> | ||
</div> | </div> | ||
| - | <p>As we conceived,our new bacteria need to have appropriate defences.Generally, defence can be classified as active defence and passive defence. If we make our engineered bacteria defend actively, they will expend large amounts of energy fighting against the phages even in an environment without phages. If so, the production of fermented products will be affected. For this reason, we successfully design our bacteria which can detect the infection of the phages. Only when the phage infects the host, the host will start its resistance against the phage.</p> | + | <p>As we conceived,our new bacteria need to have appropriate defences in order to prevent the whole population from phages.Generally, defence can be classified as active defence and passive defence. If we make our engineered bacteria defend actively, they will expend large amounts of energy fighting against the phages even in an environment without phages. If so, the production of fermented products will be affected. For this reason, we successfully design our bacteria which can detect the infection of the phages. Only when the phage infects the host, the host will start its resistance against the phage.</p> |
<p>We kill the phage by means of making the host suicide. Provided that the host can suicide fast enough and die before newly assembled phages become mature, the new waves of infection will be successfully avoided. In this case, the damage the phages can cause will be much lower. What’s more, our engineered bacteria can efficiently kill the lysogenic bacteria which hide in the colonies. This will guarantee the colony’s safety from the origin.</p> | <p>We kill the phage by means of making the host suicide. Provided that the host can suicide fast enough and die before newly assembled phages become mature, the new waves of infection will be successfully avoided. In this case, the damage the phages can cause will be much lower. What’s more, our engineered bacteria can efficiently kill the lysogenic bacteria which hide in the colonies. This will guarantee the colony’s safety from the origin.</p> | ||
<p>However, the phage is still possible to release plenty of mature phages before the host suicides. What’s worse, the circumstance in the fermentation tanks is very beneficial for bacteria to grow. And when a lysogenic bacterium lives in a fertile circumstance, the phage tend to transform into lytic life cycle.(See the introduction about lambda phage and its lysogenic and lytic life cycle). In order to prevent the phage from reproducing and releasing large amounts of newly assembled phages, we must decrease the possibility for the phage to turn into the lytic life cycle and make it stay at lysogenic life cycle. In this case, we can win plenty of time for this bacterium to defeat the phage.</p> | <p>However, the phage is still possible to release plenty of mature phages before the host suicides. What’s worse, the circumstance in the fermentation tanks is very beneficial for bacteria to grow. And when a lysogenic bacterium lives in a fertile circumstance, the phage tend to transform into lytic life cycle.(See the introduction about lambda phage and its lysogenic and lytic life cycle). In order to prevent the phage from reproducing and releasing large amounts of newly assembled phages, we must decrease the possibility for the phage to turn into the lytic life cycle and make it stay at lysogenic life cycle. In this case, we can win plenty of time for this bacterium to defeat the phage.</p> | ||
Revision as of 12:33, 5 September 2012
General IdeasOur Defences Designed
Some description of the picture
As we conceived,our new bacteria need to have appropriate defences in order to prevent the whole population from phages.Generally, defence can be classified as active defence and passive defence. If we make our engineered bacteria defend actively, they will expend large amounts of energy fighting against the phages even in an environment without phages. If so, the production of fermented products will be affected. For this reason, we successfully design our bacteria which can detect the infection of the phages. Only when the phage infects the host, the host will start its resistance against the phage.
We kill the phage by means of making the host suicide. Provided that the host can suicide fast enough and die before newly assembled phages become mature, the new waves of infection will be successfully avoided. In this case, the damage the phages can cause will be much lower. What’s more, our engineered bacteria can efficiently kill the lysogenic bacteria which hide in the colonies. This will guarantee the colony’s safety from the origin.
However, the phage is still possible to release plenty of mature phages before the host suicides. What’s worse, the circumstance in the fermentation tanks is very beneficial for bacteria to grow. And when a lysogenic bacterium lives in a fertile circumstance, the phage tend to transform into lytic life cycle.(See the introduction about lambda phage and its lysogenic and lytic life cycle). In order to prevent the phage from reproducing and releasing large amounts of newly assembled phages, we must decrease the possibility for the phage to turn into the lytic life cycle and make it stay at lysogenic life cycle. In this case, we can win plenty of time for this bacterium to defeat the phage.
At the same time, to keep the safety of the colony better, we use the quorum sensing system to make the bacteria around the host start to defend. But due to the complexity of the E.coli’s membrane, we failed to find methods to prevent the phages from invading into the bacteria. Also because of deficiency of time, we did not complete the quorum sensing system.
Maybe the second stageAs I thought before
Some description of the picture
Here at Koodoz Design, we create custom-designed websites that are unique and engaging, meet the needs of your business and target your clients and potential clients.
All of our sites are handcrafted with clean, standards-compliant code, using strict (x)HTML and CSS to ensure your site not only looks great, but performs well too.
