Team:Macquarie Australia/Protocols/GibsonAssembly
From 2012.igem.org
(Difference between revisions)
Line 18: | Line 18: | ||
! scope="col"| #5A | ! scope="col"| #5A | ||
|- | |- | ||
- | ! scope="row"| Addition of 20ng Gene Block Fragments in appropriate tube | + | ! scope="row"| 1. Addition of 20ng Gene Block Fragments in appropriate tube |
| 2µl Hemo_T7A | | 2µl Hemo_T7A | ||
| 2µl Hemo_A | | 2µl Hemo_A |
Revision as of 09:47, 5 September 2012
Gibson Assembly Protocol
Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.
Instruction | #1C | #2K | #2A | #3K | #3A | #4C | #5K | #5A |
---|---|---|---|---|---|---|---|---|
1. Addition of 20ng Gene Block Fragments in appropriate tube | 2µl Hemo_T7A | 2µl Hemo_A | 2µl Hemo_A | 2µl Deino_A | 2µl Deino_A | 2µl Agro_T7A | 2µl Agro_A | 2µl Agro_A |
2µl Hemo_B | 2µl Hemo_B | 2µl Hemo_B | 2µl Deino_B | 2µl Deino_B | 2µl Agro_B | 2µl Agro_B | 2µl Agro_B
| |
2µl Deino_C | 2µl Deino_C | 2µl Agro_C | 2µl Agro_C | 2µl Agro_C
| ||||
2µl Deino_D | 2µl Deino_D | 2µl Agro_D | 2µl Agro_D | 2µl Agro_D
| ||||
2µl Deino_E | 2µl Deino_E | 2µl Agro_E | 2µl Agro_E | 2µl Agro_E | ||||
|