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Team:Macquarie Australia/Protocols/GibsonAssembly
From 2012.igem.org
(Difference between revisions)
| Line 20: | Line 20: | ||
! scope="row"| Addition of 20ng Gene Block Fragments in appropriate tube | ! scope="row"| Addition of 20ng Gene Block Fragments in appropriate tube | ||
| 2µl Hemo_T7A | | 2µl Hemo_T7A | ||
| - | | | + | | 2µl Hemo_A |
| + | | 2µl Hemo_A | ||
| + | | 2µl Deino_A | ||
| + | | 2µl Deino_A | ||
| + | | 2µl Agro_T7A | ||
| + | | 2µl Agro_A | ||
| + | | 2µl Agro_A | ||
| + | |||
|- | |- | ||
! scope="row"| | ! scope="row"| | ||
Revision as of 09:38, 5 September 2012
Gibson Assembly Protocol
Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.
| Instruction | #1C | #2K | #2A | #3K | #3A | #4C | #5K | #5A |
|---|---|---|---|---|---|---|---|---|
| Addition of 20ng Gene Block Fragments in appropriate tube | 2µl Hemo_T7A | 2µl Hemo_A | 2µl Hemo_A | 2µl Deino_A | 2µl Deino_A | 2µl Agro_T7A | 2µl Agro_A | 2µl Agro_A |
| 2µl Hemo_B | ||||||||
|
| ||||||||
|
    |
