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Revision as of 09:32, 5 September 2012 (view source) Ehoyer (Talk | contribs) ← Older edit		Revision as of 09:33, 5 September 2012 (view source) Ehoyer (Talk | contribs) Newer edit →
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	! scope="col"| #5A		! scope="col"| #5A
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-	! scope="row"| T7 Heme oxygenase	+	! scope="row"| Addition of 20ng Gene Block Fragments in appropriate tube
	| Hemo_T7_A + Hemo_B + Plasmid		| Hemo_T7_A + Hemo_B + Plasmid
	|-		|-

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Revision as of 09:33, 5 September 2012

Gibson Assembly Protocol

Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.

Instruction	#1C	#2K	#2A	#3K	#3A	#4C	#5K	#5A
Addition of 20ng Gene Block Fragments in appropriate tube	Hemo_T7_A + Hemo_B + Plasmid
Heme oxygenase (T7 minus)	Hemo_A + Hemo_B + Plasmid
T7 Agrobacterium	Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus)	Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid Performing Gibson Assembly Planning lab work for the day Calculations