Team:Macquarie Australia/Protocols/GibsonAssembly

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Revision as of 09:32, 5 September 2012



Gibson Assembly Protocol

Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.

Instruction #1C #2K #2A #3K #3A #4C #5K #5A
T7 Heme oxygenase Hemo_T7_A + Hemo_B + Plasmid
Heme oxygenase (T7 minus) Hemo_A + Hemo_B + Plasmid
T7 Agrobacterium Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus) Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid





Performing Gibson Assembly
Planning lab work for the day
Calculations
IMG 6152.JPG