Team:Macquarie Australia/Protocols/GibsonAssembly

From 2012.igem.org

(Difference between revisions)
(→Gibson Assembly Protocol)
(→Gibson Assembly Protocol)
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Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.
Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.
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Calculations:
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{| class="wikitable"
 +
|-
 +
! scope="col"|
 +
! scope="col"| #1C
 +
! scope="col"| #2K
 +
! scope="col"| #2A
 +
! scope="col"| #3K
 +
! scope="col"| #3A
 +
! scope="col"| #4C
 +
! scope="col"| #5K
 +
! scope="col"| #5A
 +
|-
 +
! scope="row"| T7 Heme oxygenase
 +
| Hemo_T7_A + Hemo_B + Plasmid
 +
|-
 +
! scope="row"| Heme oxygenase (T7 minus)
 +
| Hemo_A + Hemo_B + Plasmid
 +
|-
 +
! scope="row"| T7 Agrobacterium
 +
| Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
 +
|-
 +
! scope="row"| Agrobacterium (T7 minus)
 +
| Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
[[File:IMG 6181.JPG|325 px|thumb|center|Performing Gibson Assembly]]
[[File:IMG 6181.JPG|325 px|thumb|center|Performing Gibson Assembly]]

Revision as of 09:31, 5 September 2012



Gibson Assembly Protocol

Before entering the lab, the assigned Gibson Assembly team for the day organised agar plate requirements, concentration calculations and equipment requirements.

#1C #2K #2A #3K #3A #4C #5K #5A
T7 Heme oxygenase Hemo_T7_A + Hemo_B + Plasmid
Heme oxygenase (T7 minus) Hemo_A + Hemo_B + Plasmid
T7 Agrobacterium Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid
Agrobacterium (T7 minus) Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid




Performing Gibson Assembly
Planning lab work for the day
Calculations
IMG 6152.JPG
Retrieved from "http://2012.igem.org/Team:Macquarie_Australia/Protocols/GibsonAssembly"