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Team:Tokyo Tech/Projects/LasR generator/index.htm
From 2012.igem.org
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| - | 1.Prepare overnight culture at | + | 1.Prepare overnight culture at 37 degrees for 12hours. (2 tubes for each sample) |
2.Take 30 μl of the overnight culture of reporter cell into LB + antibiotics (Amp + Kan). (→fresh culture) | 2.Take 30 μl of the overnight culture of reporter cell into LB + antibiotics (Amp + Kan). (→fresh culture) | ||
3.Incubate the flesh culture of reporter cell] until the observed O.D. reaches around 0.60. and gather the supernatant of culture of inducer cell. | 3.Incubate the flesh culture of reporter cell] until the observed O.D. reaches around 0.60. and gather the supernatant of culture of inducer cell. | ||
Revision as of 06:25, 4 September 2012
LasR generator
Abstract
We succeeded in constructing and characterizing a NEW Biobrick device (name). This device is a LasR generator We found that the device is (explain)
Protocol
1.Prepare overnight culture at 37 degrees for 12hours. (2 tubes for each sample) 2.Take 30 μl of the overnight culture of reporter cell into LB + antibiotics (Amp + Kan). (→fresh culture) 3.Incubate the flesh culture of reporter cell] until the observed O.D. reaches around 0.60. and gather the supernatant of culture of inducer cell. 4.Each sample of reporter cell was divided into 2. Prepare and add AHL mixture to one, cell-synthesized AHL to another one, and DMSO mixture to the other. 5.Induction of reporter cell for 3 hours.
6.Fluorometer (FLA5200) and flow cytometry measurements for GFP expression of reporter cell
