"
Team:Macquarie Australia/Notebook
From 2012.igem.org
| Line 116: | Line 116: | ||
===[[Team: Macquarie_Australia/Protocols/SchoolVisit | School Visit]]=== | ===[[Team: Macquarie_Australia/Protocols/SchoolVisit | School Visit]]=== | ||
| - | *'''Outreach''' | + | *'''Outreach administration''' |
| + | *'''Media preparation for more Fluorescent bacteria | ||
===[[Team: Macquarie_Australia/Protocols/CompetentCellforGibson | Competent Cell Protocol]]=== | ===[[Team: Macquarie_Australia/Protocols/CompetentCellforGibson | Competent Cell Protocol]]=== | ||
*'''Generation of Competent Cells''' | *'''Generation of Competent Cells''' | ||
| - | |||
| - | |||
| - | |||
| - | |||
Revision as of 01:51, 4 September 2012
Contents |
Tuesday 7/8/12
(Click on headings to visit methods)
Making liquid media, plates & buffers
- Liquid LB Media
- SOC Solution
- SOB Solution
- LB Agar Plates
Ampicillin LB Agar Plates: 31 plates
Chloramphenicol LB Agar Plates: 33 Plates
Kanamyacin LB Agar Plates: 32 Plates
- TB buffer.
- TAE buffer.
- EDTA buffer.
Designing Gibson Assembly Fragments
- Haemoxygenase Gene
- Deinococcus radiodurans Bacteriophytochrome Gene
- Agrobacterium tumefaciens Bacteriophytochrome Gene
Tuesday 14/8/12
(Click on headings to visit methods)
Making Competent Cells
- Choosing Biobricks
- Making competent cells
- Transformations
Outreach Planning Labwork
- Open Day
- Secondary Education Seminars
Wednesday 15/8/12
Our open day preparation did not go as planned. Most of our transformations were unsuccessful, we believe this was because SOC was added after the heat shock. As disappointing as this was, there was one little piece of good news.
A single colony of Top 10 E.coli containing GFP insert was identified on a plate of LB agar media.
LB agar and nutrient agar plates were struck using this colony. The colony was also used to inoculate 5mL of nutrient broth. Plates and stock were then incubated at 37°C overnight. Both of the streaked plates and the broth showed significant growth of E.coli colonies which could fluoresce when exposed to UV light.
Unfortunately, Qantas declined to sponsor the effort of getting our team to Hong Kong. This was soon forgotten when the Macquarie Faculty of Medicine, Agilent and the Defence Science Institute (thanks Yagiz) both agreed to sponsor our team. Hong Kong here we come.
Tuesday 21/8/12
Outreach Planning Labwork
- Secondary education seminars
- Reattempting glowing bacteria
Making Plates
Tuesday 28/8/12
Outreach Planning
- High School Presentations: Preparation for 4th September
- Open Day: Preparation for 8th September at Macquarie University
- Writing the Abstract for the project due 7th September
Outreach Lab Work
- Fluorescent Bacteria
Competent Cell Testing
Friday 31/8/12
gBlock fragments
- gBlock fragments from IDT
Tuesday 04/09/12
Gibson Assembly
- Gibson Assembly Protocol
School Visit
- Outreach administration
- Media preparation for more Fluorescent bacteria
Competent Cell Protocol
- Generation of Competent Cells
