Team:TU Darmstadt/Protocols
From 2012.igem.org
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<li><a href="/Team:TU_Darmstadt/Project" title="Project">Project</a><ul> | <li><a href="/Team:TU_Darmstadt/Project" title="Project">Project</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Project" title="Team:Overview">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Project" title="Team:Overview">Overview</a></li> | ||
- | <li><a href="/Team:TU_Darmstadt/Degradation" title="Degradation">1. Degradation</a></li> | + | <li><a href="/Team:TU_Darmstadt/Project/Degradation" title="Degradation">1. Degradation</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Transport" title="Transport">2. Transport</a></li> | + | <li><a href="/Team:TU_Darmstadt/Project/Transport" title="Transport">2. Transport</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Metabolism" title="Metabolism">3. Metabolism</a></li> | + | <li><a href="/Team:TU_Darmstadt/Project/Metabolism" title="Metabolism">3. Metabolism</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Material_Science" title="Material Science">4. Material Science</a></li> | + | <li><a href="/Team:TU_Darmstadt/Project/Material_Science" title="Material Science">4. Material Science</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Simulation" title="Simulation">5. Simulation</a></li> | + | <li><a href="/Team:TU_Darmstadt/Project/Simulation" title="Simulation">5. Simulation</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Ecology" title="Ecology">Ecology</a></li> | + | <li><a href="/Team:TU_Darmstadt/Project/Ecology" title="Ecology">Ecology</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Philosophy" title="Philosophy">Philosophy</a></li></ul></li> | + | <li><a href="/Team:TU_Darmstadt/Project/Philosophy" title="Philosophy">Philosophy</a></li></ul></li> |
<li><a href="/Team:TU_Darmstadt/Team" title="Team:TU_Darmstadt/Team">Team</a><ul> | <li><a href="/Team:TU_Darmstadt/Team" title="Team:TU_Darmstadt/Team">Team</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Team" title="Team:TU_Darmstadt/Team">Members</a></li> | <li><a href="/Team:TU_Darmstadt/Team" title="Team:TU_Darmstadt/Team">Members</a></li> | ||
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<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul> | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul> | ||
- | <li><a href="/Team:TU_Darmstadt/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li> | + | <li><a href="/Team:TU_Darmstadt/Human_Practice/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Symposia" title="Symposia">Symposia</a></li> | + | <li><a href="/Team:TU_Darmstadt/Human_Practice/Symposia" title="Symposia">Symposia</a></li> |
- | <li><a href="/Team:TU_Darmstadt/Classes" title="Classes">Classes</a></li></ul></li> | + | <li><a href="/Team:TU_Darmstadt/Human_Practice/Classes" title="Classes">Classes</a></li></ul></li> |
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> |
Revision as of 19:36, 3 September 2012
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- In-fusion BioBrick assembly
- Miniprep / Qiagen kit
- Transformation of chemically competent cells
- Western Blot
Comamonas testosteroni
In vitro
Nucleic acids
DNA
- Agarose gel electrohporesis
- Annealing and primer extension
- Annealing primers
- Assembly PCR
- Cloning
- DNA Ligation
- DNA Quantification / NanoDrop
- DNA Synthesis from Oligos
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Miniprep
- PCR
- Polyacrylamide gel electrophoresis
- Primer Design
- Purification of DNA
- Restriction digest
- Resuspension of primers
- Salting Out
- Sequencing DNA
RNA
Protein
In silico
Data analysis
Databases
Modelling
Sequence analysis
Structure analysis
Miscellaneous
Cell culture
Microscopy
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers