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Team:LMU-Munich/Weekly Journal
From 2012.igem.org
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| - | ''' | + | '''06-09 August 2012''' |
<html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"> | <html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"> | ||
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Created double-mutant using resistance-cassette KOs: cwlD::kan+gerD::cat | Created double-mutant using resistance-cassette KOs: cwlD::kan+gerD::cat | ||
| - | ''' | + | '''30 July - 10 August 2012''' |
| - | ''' | + | '''23-27 July 2012''' |
| + | Plate reader measurements of the Bacillus promoters PliaG, PliaI and PlepA finished. Look at Data! | ||
| - | ''' | + | '''16-20 July 2012''' |
| + | |||
| + | Plate reader measurements of the Anderson promoters J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 in the ''Bacillus'' reporter vector pSBBs3C-luxABCDE completed. Look at Data! | ||
| + | |||
| + | Cloning of the Anderson promoters J23100, J23102, J23103, J23106 in the reporter vector pSBBs1C-''lacZ'' for β-galactosidase assays finished. | ||
| - | + | '''9-13 July 2012''' | |
| - | ''' | + | |
| - | ''' | + | '''02-06 July 2012''' |
<html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"> | <html><a href="https://2012.igem.org/Team:LMU-Munich/Germination_Stop"> | ||
<img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=40"/></a></html> | <img src="https://static.igem.org/mediawiki/2012/f/f6/GerminationSTOP.png" height=40"/></a></html> | ||
Jenny presented ''Bacillus'' germination review to the group. We discussed stages of germination & how our mutants would prevent germination from occurring. Discussed possible germination causes besides nutrients, and how to hopefully prevent them. | Jenny presented ''Bacillus'' germination review to the group. We discussed stages of germination & how our mutants would prevent germination from occurring. Discussed possible germination causes besides nutrients, and how to hopefully prevent them. | ||
| + | |||
| + | β-galactosidase assays of the promoters PliaG, PliaI and Pveg are finished. Look at Data! | ||
| + | |||
| + | |||
| + | Cloning of the Anderson promoters J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 in pSB1C3 finished. | ||
| + | |||
'''24-28 June 2012''' | '''24-28 June 2012''' | ||
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'''21-25 May 2012''' | '''21-25 May 2012''' | ||
| - | Promoters P'' | + | Promoters P''liaG'', P''liaI'', P''veg'' and P''lepA'' are now in the vector pSB1C3 as BioBrick standard for the registry. |
The Anderson promoters J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 are now in the ''Bacillus'' reporter vector pSBBs3C-luxABCDE for measuring their activity as luminescence. | The Anderson promoters J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 are now in the ''Bacillus'' reporter vector pSBBs3C-luxABCDE for measuring their activity as luminescence. | ||
Revision as of 11:08, 3 September 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Weekly Journal
Legend:
| +--+--+--+--+--+--+-- | +--+--+--+--+-- | +--+--+--+--+--+-- | +--+--+--+--+--+-- |
Bacillus BioBrickBOX |
SporeCoat FusionProteins |
Germination STOP |
26-30 August 2012
Finally, we got our first glowing spore!! After 4 month of hard work we have our first proof, that our system works. 
19-23 August 2012
Double negative loop with lacZa finished -> works qualitatively (Julia)
12-16 August 2012
06-09 August 2012
Created double-mutant using resistance-cassette KOs: cwlD::kan+gerD::cat
30 July - 10 August 2012
23-27 July 2012
Plate reader measurements of the Bacillus promoters PliaG, PliaI and PlepA finished. Look at Data!
16-20 July 2012
Plate reader measurements of the Anderson promoters J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 in the Bacillus reporter vector pSBBs3C-luxABCDE completed. Look at Data!
Cloning of the Anderson promoters J23100, J23102, J23103, J23106 in the reporter vector pSBBs1C-lacZ for β-galactosidase assays finished.
9-13 July 2012
02-06 July 2012
Jenny presented Bacillus germination review to the group. We discussed stages of germination & how our mutants would prevent germination from occurring. Discussed possible germination causes besides nutrients, and how to hopefully prevent them.
β-galactosidase assays of the promoters PliaG, PliaI and Pveg are finished. Look at Data!
Cloning of the Anderson promoters J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 in pSB1C3 finished.
24-28 June 2012
17- 21 June 2012
10-14 June 2012
3-7 June 2012
27-31 May 2012
21-25 May 2012
Promoters PliaG, PliaI, Pveg and PlepA are now in the vector pSB1C3 as BioBrick standard for the registry.
The Anderson promoters J23100, J23101, J23102, J23103, J23106, J23107, J23113, J23114, J23115, J23117, J23118 are now in the Bacillus reporter vector pSBBs3C-luxABCDE for measuring their activity as luminescence.
The Bacillus promoters PliaG, PliaI and PlepA are in the Bacillus reporter vector pSBBs3C-luxABCDE.
13-17 May 2012
7-11 May 2012
Cloning of PliaG, PliaI and Pveg in vector pSBBs1C-lacZ finished.
30 April-4 Mai 2012
22-26 April 2012
15-19 April 2012
8-12 April 2012
1-5 April 2012
Jara finished resistance-cassette KOs of genes: cwlD, cwlJ, sleB, and gerD.
25-29 March 2012
18- 22 March 2012
11-15 March 2012
4-8 March 2012
26 February - 1 March 2012
19-23 February 2012
Team fully formed!
