Team:Potsdam Bioware

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*<b>"Go, Krauts, go"</b> - Best german team 2012 is Potsdam (same like last year) :)!  
*<b>"Go, Krauts, go"</b> - Best german team 2012 is Potsdam (same like last year) :)!  

Revision as of 22:44, 31 August 2012

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Direct Antibody Identification, Maturation and Production in CHO-Cells

Project Description

Antibodies are versatile molecules for research and therapy and they are currently revolutionizing the market of biopharmaceuticals. To date, antibodies are generated in a laborious and time consuming manner. Either animal immunization followed by hybridoma technology or phage display is used to identify desired genes, which then need to be transferred to a production cell line such as CHO. We designed a streamlined workflow that incorporates all steps of antibody generation in CHO cells. Our plan is to stably transfect an antibody-fragment library in CHO cells. Antibody maturation is mimicked by further diversifying this library using the enzyme Activation-Induced cytidine Deaminase (AID), which is known to induce somatic hypermutation. Next, we plan to test and deploy a versatile and continuous viral selection system for clones producing the desired antibodies. Finally, we will try to employ a genetic switch to go from surface expression to soluble production of antibodies.

  • "Go, Krauts, go" - Best german team 2012 is Potsdam (same like last year) :)!


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Team

Mario Köhler
Tom Scheidt
Christopher Kressler
Tobias Priemel
Maria Kunert
Sascha Lehnort
Norman Hein
Retrieved from "http://2012.igem.org/Team:Potsdam_Bioware"