"
Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
(Difference between revisions)
| Line 28: | Line 28: | ||
|- | |- | ||
! scope="row"| Agrobacterium (T7 minus) | ! scope="row"| Agrobacterium (T7 minus) | ||
| - | | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E | + | | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
|} | |} | ||
Revision as of 08:53, 31 August 2012
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.
| BioBrick name | Fragment(s) |
|---|---|
| T7 Heme oxygenase | Hemo_T7_A + Hemo_B + Plasmid |
| Heme oxygenase (T7 minus) | Hemo_A + Hemo_B + Plasmid |
| T7 Agrobacterium | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
| Agrobacterium (T7 minus) | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter. The designed sequences of the 5 fragments can be viewed here
| BioBrick name | Fragment(s) |
|---|---|
| Deinococcus radiodurans bacteriophytochrome | Deino_A + Deino_B + Deino_C + Deino_D + Deino_E + Plasmid |
