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Team:Macquarie Australia/Protocols/ArrivalofGBlocks
From 2012.igem.org
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| - | Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter. | + | Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter. The designed sequences of the 5 fragments can be viewed[[media:DeinoA.pdf | here ]] |
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! scope="row"| Deinococcus radiodurans bacteriophytochrome | ! scope="row"| Deinococcus radiodurans bacteriophytochrome | ||
| - | | Deino_A + Deino_B + Deino_C + Deino_D + Deino_E | + | | Deino_A + Deino_B + Deino_C + Deino_D + Deino_E + Plasmid |
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Revision as of 08:42, 31 August 2012
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.
| BioBrick name | Fragment(s) |
|---|---|
| T7 Heme oxygenase | Hemo_T7_A + Hemo_B + Plasmid |
| Heme oxygenase (T7 minus) | Hemo_A + Hemo_B + Plasmid |
| T7 Agrobacterium | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
| Agrobacterium (T7 minus) | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E |
Once the Deinococcus Radiodurans fragments arrive we will assemble them using Gibson assembly. Due to the large sequence of the D. radiodurans bacteriophytochrome, the fragments will be assembled without a T7 promoter. The designed sequences of the 5 fragments can be viewed here
| BioBrick name | Fragment(s) |
|---|---|
| Deinococcus radiodurans bacteriophytochrome | Deino_A + Deino_B + Deino_C + Deino_D + Deino_E + Plasmid |
