Team:Macquarie Australia/Protocols/ArrivalofGBlocks
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(Difference between revisions)
Line 23: | Line 23: | ||
! scope="row"| Heme oxygenase (T7 minus) | ! scope="row"| Heme oxygenase (T7 minus) | ||
| Hemo_A + Hemo_B + Plasmid | | Hemo_A + Hemo_B + Plasmid | ||
- | + | |- | |
! scope="row"| T7 Agro bacterium | ! scope="row"| T7 Agro bacterium | ||
| Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid | | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
Revision as of 08:27, 31 August 2012
Ordered gBlock fragments are nearly here!
Soon our gBlock fragments will arrive from IDT. On Tuesday (4/09/2012) we will perform Gibson assembly will assemble the following four biobricks:
- +T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- -T7 promoter Agrobacterium tumefaciens bacteriophytochrome
- +T7 promoter Heme oxygenase
- -T7 promoter Heme oxygenase
Click on each above to see the corresponding fragment nucleotide sequence and fragment properties.
BioBrick name | Fragment(s) |
---|---|
T7 Heme oxygenase | Hemo_T7_A + Hemo_B + Plasmid |
Heme oxygenase (T7 minus) | Hemo_A + Hemo_B + Plasmid |
T7 Agro bacterium | Agro_T7_A + Agro_B + Agro_C + Agro_D + Agro_E + Plasmid |
Agrobacterium (T7 minus) | Agro_A + Agro_B + Agro_C + Agro_D + Agro_E |