Utk knoxville august

From 2012.igem.org

(Difference between revisions)
(Created page with "8.25.12 8.26.12 Amplified GFP + Cyc. 8.28.12 Digested : J63010 + Adh with EcoRI and PstI to confirm construct. J63010 + Adh with SpeI and PstI. PSB1C3+ KanR with XbaI an...")
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8.25.12  
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8.20.12  
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Digested PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc with EcoRI & PstI for confirmation.
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Digested  J63010 + Adh + mOrange with SpeI and PstI. Digested J63010 + pIRES + GFP + cyc with XbaI and PstI.
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8.21.12
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Ran gel of check digestions for PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc. All three constructs were confirmed.
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Ligated pIRES + GFP + cyc into J63010 + Adh + mOrange. Did 1:0, 1:10, and 1:30.
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Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc.
8.26.12  
8.26.12  
Amplified GFP + Cyc.  
Amplified GFP + Cyc.  
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Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc.
8.28.12  
8.28.12  

Revision as of 20:20, 30 August 2012

8.20.12 Digested PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc with EcoRI & PstI for confirmation. Digested J63010 + Adh + mOrange with SpeI and PstI. Digested J63010 + pIRES + GFP + cyc with XbaI and PstI.


8.21.12 Ran gel of check digestions for PSB1C3 + Yap + GFP + cyc, PSB1C3 + URE2 + GFP + cyc, and PSB1C3 + HAP + GFP + cyc. All three constructs were confirmed. Ligated pIRES + GFP + cyc into J63010 + Adh + mOrange. Did 1:0, 1:10, and 1:30. Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc.

8.26.12 Amplified GFP + Cyc. Performed colony PCR on J63010 + Adh + mOrange + pIRES + GFP + cyc.

8.28.12 Digested : J63010 + Adh with EcoRI and PstI to confirm construct. J63010 + Adh with SpeI and PstI. PSB1C3+ KanR with XbaI and PstI. PSB1C3 + p150 with SpeI and PstI. Amplified GFP + Cyc with XbaI and PstI.