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Only using half the amount of cells from the original protocol, only using half the solution amount. | Only using half the amount of cells from the original protocol, only using half the solution amount. | ||
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4. Not enough sol. A + glycerol for the second tube. Only ~27ml instead of 30ml. | 4. Not enough sol. A + glycerol for the second tube. Only ~27ml instead of 30ml. | ||
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Forgot to insert protocol.
Only using half the amount of cells from the original protocol, only using half the solution amount.
2. Optical density measured at 460.
4. Not enough sol. A + glycerol for the second tube. Only ~27ml instead of 30ml.
"
