"
Team:Grenoble/Biology/Protocols/Glycerol
From 2012.igem.org
(Difference between revisions)
| Line 14: | Line 14: | ||
You need to: | You need to: | ||
<ol> | <ol> | ||
| - | <li> | + | <li>Grow a liquid culture overnight</li> |
| - | <li> | + | <li>Prepare sterile 80% glycerol</li> |
| - | <li> | + | <li>Mix 200 µL of glycerol and 800 µL of liquid culture</li> |
</ol> | </ol> | ||
<br/> | <br/> | ||
Revision as of 14:20, 27 August 2012
Glycerol stock preparation
Goal
Prepare glycerol stock is a way to store bacteria during a long period.Protocol
You need to:- Grow a liquid culture overnight
- Prepare sterile 80% glycerol
- Mix 200 µL of glycerol and 800 µL of liquid culture
Then store the glycerol stock at -80°C.
