"
Team:Cambridge/Protocols/biobrick protocols
From 2012.igem.org
(Difference between revisions)
CharlotteBG (Talk | contribs) |
(→Resuspension of BioBricks from the distribution) |
||
| Line 13: | Line 13: | ||
#Pierce foil of required well and pipette 10µL dH<sub>2</sub>O (distilled water). | #Pierce foil of required well and pipette 10µL dH<sub>2</sub>O (distilled water). | ||
#Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes. | #Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes. | ||
| - | #The BioBrick is now ready for | + | #The BioBrick is now ready for [[Team:Cambridge/Protocols/TransformationofE.coli| transformation]] |
==Linearised Plasmid Backbones== | ==Linearised Plasmid Backbones== | ||
Revision as of 20:56, 23 August 2012
BioBricks
[http://partsregistry.org/Help:Distribution_Kits Distribution Kit page] A link to the Registry of Standard Biological Parts
Resuspension of BioBricks from the distribution
see [http://partsregistry.org/Help:Distribution_Kits#Using_the_Spring_2012_DNA_Distribution Using the Spring 2012 DNA Distribution] for a more detailed protocol!
- Locate your BioBrick of interest in the Distribution: [http://partsregistry.org/assembly/libraries.cgi?id=42 Spring 2012 Distribution, DNA Part Repositories] Make sure the plate is properly orientated! The two notched corners of the plate are oriented at the BOTTOM of the plate.
- Pierce foil of required well and pipette 10µL dH2O (distilled water).
- Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes.
- The BioBrick is now ready for transformation
Linearised Plasmid Backbones
[http://partsregistry.org/Help:Protocols/Linearized_Plasmid_Backbones Linearised Plasmid Backbone]
