Team:Technion/23 August 2012
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The bacteria grew on the negative control (no insert) plates… <br> | The bacteria grew on the negative control (no insert) plates… <br> | ||
- Colony PCR for: <br> | - Colony PCR for: <br> | ||
- | + | Six positive colonies (pSB1C3+MCS) <br> | |
- | + | Two negative control colonies (pSB1C3 without insert?!) <br> | |
- | + | Positive control (pSB1C3 + Fus_1) <br> | |
- | + | Negative control (no colony inserted) <br> | |
- 1% gel for the PCR products. <br> | - 1% gel for the PCR products. <br> | ||
- | + | I got five positive colonies (number 1-4, 6) with the wanted 344 bp band. <br> | |
- | + | Colony number 5 showed a 1106 bp band (consider negative for the ligation) like the negative control colonies and the positive control for the experiment. <br> | |
We think that the CIP enzyme was contaminated so the reaction was not as good as it should… | We think that the CIP enzyme was contaminated so the reaction was not as good as it should… | ||
- | + | No bend appeared in the negative control strip. <br> | |
Revision as of 14:47, 23 August 2012
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Ilya
Inbal
Asaf
Hila
The bacteria grew on the negative control (no insert) plates…
- Colony PCR for:
Six positive colonies (pSB1C3+MCS)
Two negative control colonies (pSB1C3 without insert?!)
Positive control (pSB1C3 + Fus_1)
Negative control (no colony inserted)
- 1% gel for the PCR products.
I got five positive colonies (number 1-4, 6) with the wanted 344 bp band.
Colony number 5 showed a 1106 bp band (consider negative for the ligation) like the negative control colonies and the positive control for the experiment.
We think that the CIP enzyme was contaminated so the reaction was not as good as it should…
No bend appeared in the negative control strip.