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Team:Cambridge/Protocols/biobrick protocols
From 2012.igem.org
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===Resuspension of BioBricks from the distribution=== | ===Resuspension of BioBricks from the distribution=== | ||
| - | [http://partsregistry.org/Help:Distribution_Kits#Using_the_Spring_2012_DNA_Distribution Using the Spring 2012 DNA Distribution] | + | see [http://partsregistry.org/Help:Distribution_Kits#Using_the_Spring_2012_DNA_Distribution Using the Spring 2012 DNA Distribution] for a more detailed protocol! |
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| + | #Locate your BioBrick of interest in the Distribution: [http://partsregistry.org/assembly/libraries.cgi?id=42 Spring 2012 Distribution, DNA Part Repositories] Make sure the plate is properly orientated! The two notched corners of the plate are oriented at the BOTTOM of the plate. | ||
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| + | #Pierce foil of required well and pipette 10µL dH<sub>2</sub>O (distilled water). | ||
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| + | #Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes. | ||
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| + | #The BioBrick is now ready for transformation. | ||
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{{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_FOOT}} | ||
Revision as of 13:19, 21 August 2012
BioBricks
[http://partsregistry.org/Help:Distribution_Kits Distribution Kit page] A link to the Registry of Standard Biological Parts
Resuspension of BioBricks from the distribution
see [http://partsregistry.org/Help:Distribution_Kits#Using_the_Spring_2012_DNA_Distribution Using the Spring 2012 DNA Distribution] for a more detailed protocol!
- Locate your BioBrick of interest in the Distribution: [http://partsregistry.org/assembly/libraries.cgi?id=42 Spring 2012 Distribution, DNA Part Repositories] Make sure the plate is properly orientated! The two notched corners of the plate are oriented at the BOTTOM of the plate.
- Pierce foil of required well and pipette 10µL dH2O (distilled water).
- Pipette up and down several times to fully resuspend the DNA sample. Sit for 10 minutes.
- The BioBrick is now ready for transformation.
