"
Team:Macquarie Australia/Notebook
From 2012.igem.org
(Difference between revisions)
| Line 64: | Line 64: | ||
=Thursday 16/8/12= | =Thursday 16/8/12= | ||
Our open day preparation did not originally go as planned. Of all our constructs (Green, orange, cyan, red fluorescent protein) only one GFP colony was grown. This was subcultured onto nutrient agar and ampicilin, a broth was also innoculated. This was observed under UV-light- it was fluorescent! We decided to continue with this inoculate. | Our open day preparation did not originally go as planned. Of all our constructs (Green, orange, cyan, red fluorescent protein) only one GFP colony was grown. This was subcultured onto nutrient agar and ampicilin, a broth was also innoculated. This was observed under UV-light- it was fluorescent! We decided to continue with this inoculate. | ||
| + | |||
| + | ---- | ||
| + | |||
| + | =Tuesday 21/8/12= | ||
| + | |||
| + | ===[[Team:Macquarie_Australia/Protocols/Outreach Planning |Outreach Planning Labwork ]]=== | ||
| + | |||
| + | *'''Glowing fruit''' | ||
| + | *'''Secondary education seminars''' | ||
| + | |||
| + | ===[[Team:Macquarie_Australia/Protocols/Biobrick labwork |Biobrick Labwork ]]=== | ||
Revision as of 00:27, 21 August 2012
Contents |
Tuesday 7/8/12
(Click on headings to visit methods)
Making liquid media, plates & buffers
- Liquid LB Media
- SOC Solution
- SOB Solution
- LB Agar Plates
Ampicillin LB Agar Plates: 31 plates
Chloramphenicol LB Agar Plates: 33 Plates
Kanamyacin LB Agar Plates: 32 Plates
- TB buffer.
- TAE buffer.
- EDTA buffer.
Designing Gibson Assembly Fragments
- Haemoxygenase Gene
- Deinococcus radiodurans Bacteriophytochrome Gene
- Agrobacterium tumefaciens Bacteriophytochrome Gene
Tuesday 14/8/12
(Click on headings to visit methods)
Making Competent Cells
- Choosing Biobricks
- Making competent cells
- Transformations
Outreach Planning Labwork
- Open Day
- Secondary Education Seminars
Thursday 16/8/12
Our open day preparation did not originally go as planned. Of all our constructs (Green, orange, cyan, red fluorescent protein) only one GFP colony was grown. This was subcultured onto nutrient agar and ampicilin, a broth was also innoculated. This was observed under UV-light- it was fluorescent! We decided to continue with this inoculate.
Tuesday 21/8/12
Outreach Planning Labwork
- Glowing fruit
- Secondary education seminars
