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Team:Technion/19 August 2012
From 2012.igem.org
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1. PCR purification for PCR products of xyIE. <br> | 1. PCR purification for PCR products of xyIE. <br> | ||
2. Restriction with XmaI + XhoI for: <br> | 2. Restriction with XmaI + XhoI for: <br> | ||
| - | < | + | <pre>a. xyIE PCR products <br> |
| - | < | + | <xmp>b. 5 of Chris's promoter plasmids <br> |
3. PCR purification for cut products of xyIE and Chris's plasmids <br> | 3. PCR purification for cut products of xyIE and Chris's plasmids <br> | ||
4. Ligation of xyIE and Chris's plasmids: 5 reactions total. <br> <br> | 4. Ligation of xyIE and Chris's plasmids: 5 reactions total. <br> <br> | ||
Revision as of 09:46, 18 August 2012
{{:Team:Technion/Template:Notebook|content=
Ilya
Inbal
Asaf
Hila
Lior
Noa
1. PCR purification for PCR products of xyIE.
2. Restriction with XmaI + XhoI for:
a. xyIE PCR products <br> <xmp>b. 5 of Chris's promoter plasmids <br> 3. PCR purification for cut products of xyIE and Chris's plasmids <br> 4. Ligation of xyIE and Chris's plasmids: 5 reactions total. <br> <br> 5. If there is time: PCR for alkaline phosphatase and running on Gel. ==Evgeni== ==Shahar== ==Rachel== }}
