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Revision as of 17:47, 2 August 2012 (view source) M.Simpson (Talk | contribs) (→Maintenance Tasks) ← Older edit		Revision as of 14:28, 17 August 2012 (view source) D.Webster (Talk | contribs) (→DNA Extraction) Newer edit →
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	=Protocols=		=Protocols=
-	==DNA Extraction==	+	==DNA Purification==
	*[[Team:Cornell/Protocols/Miniprep|Miniprep]]		*[[Team:Cornell/Protocols/Miniprep|Miniprep]]
-	*[[Team:Cornell/Protocols/Gel_purification|Gel Purification]]	+	*[[Team:Cornell/Protocols/Gel_extraction|Gel Extraction]]
	==DNA Assessment==		==DNA Assessment==

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Revision as of 14:28, 17 August 2012

Home	Team	Project	Parts	Modeling	Notebook	Protocols	Safety	Attributions

Contents 1 Protocols 1.1 DNA Purification 1.2 DNA Assessment 1.3 PCR Methods 1.4 Plasmid Insertion 1.5 Plasmid Editing 1.6 Maintenance Tasks

Protocols

DNA Purification

• Miniprep
• Gel Extraction

DNA Assessment

• Gel Electrophoresis
• DNA Quantification
• Sequencing
• PCR Clean Up

PCR Methods

• Vent PCR
• Phusion PCR
• Colony PCR

Plasmid Insertion

• Transformation of E. coli
• Transformation of Shewanella
• Conjugation

Plasmid Editing

• Digestion Reaction
• Dephosphorylation
• Ligation Reaction
• Gibson Assembly

Maintenance Tasks

• Making LB
• Making Super Optimal Broths
• Making Plates
• Autoclave
• Preparing Glycerol Stocks
• Preparing Overnight Cultures
• Preparing Electrocompetent Cells