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Team:LMU-Munich/Bacillus BioBricks
From 2012.igem.org
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Here is a list of all the vectors we cloned and used. Please note that pMAD is not in a BioBrick standard, but it is a useful tool to knock out genes inBacillus subtilis. | Here is a list of all the vectors we cloned and used. Please note that pMAD is not in a BioBrick standard, but it is a useful tool to knock out genes inBacillus subtilis. | ||
| - | For the use of our vectors, please see our [Team:LMU-Munich/Lab_Notebook/Protocols Protocols] page. A general introduction to Bacillus subtilis and its integrative vectors can be found [[Team:LMU-Munich/Bacillus Introduction|here]]. All vectors have ampicillin as <i>E. coli</i> resistance and RFP as selection marker. | + | For the use of our vectors, please see our [[Team:LMU-Munich/Lab_Notebook/Protocols|Protocols]] page. A general introduction to Bacillus subtilis and its integrative vectors can be found [[Team:LMU-Munich/Bacillus Introduction|here]]. All vectors have ampicillin as <i>E. coli</i> resistance and RFP as selection marker. |
{| | {| | ||
| - | |- | + | |- <b> |
|name | |name | ||
|E. coli res. | |E. coli res. | ||
| Line 26: | Line 26: | ||
|reference | |reference | ||
|- | |- | ||
| - | |pSB<sub>Bs</sub>1C-<i>lacZ</i> | + | |pSB<sub>Bs</sub>1C-<i>lacZ</i> </b> |
|Amp | |Amp | ||
|Cam | |Cam | ||
| Line 34: | Line 34: | ||
|? | |? | ||
|- | |- | ||
| - | |pSB<sub>Bs</sub>4S | + | |<b>pSB<sub>Bs</sub>4S </b> |
|Amp | |Amp | ||
|Spec | |Spec | ||
| Line 42: | Line 42: | ||
|? | |? | ||
|- | |- | ||
| - | |pSB<sub>Bs</sub>1C | + | |<b>pSB<sub>Bs</sub>1C </b> |
|Amp | |Amp | ||
|Cam | |Cam | ||
| Line 50: | Line 50: | ||
|? | |? | ||
|- | |- | ||
| - | |pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> | + | |<b>pSB<sub>Bs</sub>4S-P<sub><i>Xyl</i></sub> </b> |
|Amp | |Amp | ||
|Spec | |Spec | ||
| Line 58: | Line 58: | ||
|? | |? | ||
|- | |- | ||
| - | |pSB<sub>Bs</sub>3C-<i>luxABCDE</i> | + | |<b>pSB<sub>Bs</sub>3C-<i>luxABCDE</i> </b> |
|Amp | |Amp | ||
|Cam | |Cam | ||
| Line 66: | Line 66: | ||
|? | |? | ||
|- | |- | ||
| - | |pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> | + | |<b>pSB<sub>Bs</sub>0K-P<sub><i>spac</i></sub> </b> |
|Amp | |Amp | ||
|Kan | |Kan | ||
| Line 74: | Line 74: | ||
|? | |? | ||
|- | |- | ||
| - | |pSB<sub>Bs</sub>2E | + | |<b>pSB<sub>Bs</sub>2E </b> |
|Amp | |Amp | ||
|MLS | |MLS | ||
| Line 83: | Line 83: | ||
|- | |- | ||
|} | |} | ||
| + | |||
| + | The number in the vector's name codes for the insertion locus and the following letter for the <i> Bacillus subtilis </i> resistance gene according to the following table: | ||
| + | |||
| + | {| | ||
| + | |- <b> | ||
| + | |number | ||
| + | |insertion locus | ||
| + | |letter | ||
| + | |resistance </b> | ||
| + | |- | ||
| + | |0 | ||
| + | |replicative | ||
| + | |C | ||
| + | |Chloramphenicol | ||
| + | |- | ||
| + | |1 | ||
| + | |amyE (amylase) | ||
| + | |E | ||
| + | |MLS (Erythromycin + Lincomycin) | ||
| + | |- | ||
| + | |2 | ||
| + | |lacA (laccase ?) | ||
| + | |K | ||
| + | |Kanamycin | ||
| + | |- | ||
| + | |3 | ||
| + | |sacA (saccase?) | ||
| + | |S | ||
| + | |Spectinomycin | ||
| + | |- | ||
| + | |4 | ||
| + | |thrC (threonine C) | ||
| + | | | ||
| + | | | ||
| + | |- | ||
| + | |||
| + | |- | ||
| + | |} | ||
| + | |||
| + | The concentrations of the antibiotics and the insertion tests can be found in our protocol section. | ||
| + | |||
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{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} | ||
Revision as of 16:57, 15 August 2012
The LMU-Munich team is exuberantly happy about the great success at the World Championship Jamboree in Boston. Our project Beadzillus finished 4th and won the prize for the "Best Wiki" (with Slovenia) and "Best New Application Project".
[ more news ]
Bacillus BioBricks
We will create a toolbox of Bacillus BioBricks to contribute to the registry.
We would like to introduce Bacillus to the world of iGEM!!!
This Bacillus BioBrick Box contains compatible vectors and useful promoters.
Here is a list of all the vectors we cloned and used. Please note that pMAD is not in a BioBrick standard, but it is a useful tool to knock out genes inBacillus subtilis.
For the use of our vectors, please see our Protocols page. A general introduction to Bacillus subtilis and its integrative vectors can be found here. All vectors have ampicillin as E. coli resistance and RFP as selection marker.
| name | E. coli res. | B. subt. res. | insertion locus | description | derived from | reference |
| pSBBs1C-lacZ | Amp | Cam | amyE | with lacZ reporter gene | pAC6 | ? |
| pSBBs4S | Amp | Spec | thrC | empty | pDG1731 | ? |
| pSBBs1C | Amp | Cam | amyE | empty | pDG1662 | ? |
| pSBBs4S-PXyl | Amp | Spec | thrC | with Xylose-inducible promoter | pXT | ? |
| pSBBs3C-luxABCDE | Amp | Cam | sacA | with luxABCDE reporter cassette | pAH328 | ? |
| pSBBs0K-Pspac | Amp | Kan | replicative | with IPTG inducible promoter | pDG148 | ? |
| pSBBs2E | Amp | MLS | lacA | empty | pAX01 | ? |
The number in the vector's name codes for the insertion locus and the following letter for the Bacillus subtilis resistance gene according to the following table:
| number | insertion locus | letter | resistance |
| 0 | replicative | C | Chloramphenicol |
| 1 | amyE (amylase) | E | MLS (Erythromycin + Lincomycin) |
| 2 | lacA (laccase ?) | K | Kanamycin |
| 3 | sacA (saccase?) | S | Spectinomycin |
| 4 | thrC (threonine C) |
The concentrations of the antibiotics and the insertion tests can be found in our protocol section.
