Team:Cambridge/Project/FluorideRiboswitch

From 2012.igem.org

(Difference between revisions)

Revision as of 13:14, 15 August 2012

Previous iGEM teams have charaterised an impressive array of inducible promoters, along with other elements of biosensing circuitry... Read More

Project

Outreach

Gallery

Diary

Team

Home

Safety

Fluoride riboswitch

We also plan on implementing and characterising a Fluoride riboswitch. This, unlike the Magnesium construct, is a positive regulator. The riboswitch, originally isolated from Bacillus cereus, serves as a transcriptional attenuator in the abscence of fluoride. In the presence of fluoride its conformation changes and the repression is lifted. In B. cereus this serves to permit translation of a fluoride efflux pump, which allows the bacteria to cope with the, potentially toxic, elevated fluoride levels in which it finds itself.

The construct we hope to make is shown below:

Our riboswitch construct for the detection of fluoride.

References

Jenny L. Baker et al., Widespread Genetic Switches and Toxicity Resistance Proteins for Fluoride, Science 335, 233-235.

Revision as of 13:12, 15 August 2012 (view source) Olijme (Talk \| contribs) (→Fluoride riboswitch) ← Older edit		Revision as of 13:14, 15 August 2012 (view source) Olijme (Talk \| contribs) (→Fluoride riboswitch) Newer edit →
Line 7:		Line 7:
	The construct we hope to make is shown below:		The construct we hope to make is shown below:

-	[[File: F-riboswitch.~~gif~~\|700px\|center\|thumb\|Our riboswitch construct for the detection of fluoride.]]	+	[[File: F-riboswitch.png\|700px\|center\|thumb\|Our riboswitch construct for the detection of fluoride.]]