Team:Macquarie Australia/Protocols/Outreach Planning
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(Created page with "For outreach we wanted to make colourful or glowing E. Coli. ::::{| border="1" cellpadding="5" cellspacing="0" |+ align="centre" style="color:#e76700;" | |- !Biobrick Name !Plas...")
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(Created page with "For outreach we wanted to make colourful or glowing E. Coli. ::::{| border="1" cellpadding="5" cellspacing="0" |+ align="centre" style="color:#e76700;" | |- !Biobrick Name !Plas...")
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Revision as of 02:34, 15 August 2012
For outreach we wanted to make colourful or glowing E. Coli.
Biobrick Name Plasmid Plate Well Promoter Gene BBa_I13521 pSB1A3 2 60 TetR repressible Red Fluorescent Protein (RFP) BBa_I13522 pSB1A2 2 A8 TetR repressible Green Fluorescent Protein (GFP) BBa_I13600 pSB1A2 1 24E TetR repressible Cyan Fluorescent Protein (CFP) BBa_I712052 pSB1AK8 2 13G T& bacteriophage Luciferase BBa_K274210 pSB1A2 3 6N TetR repressible Orange Fluorescent Protein (OFP)
1 x 200 μl on plates with added IPTG
1 x 20 μl on plates without IPTG
1 x 200 μl on plates without IPTG
The plates were then incubated overnight at 37o C.