Team:Macquarie Australia/Notebook

From 2012.igem.org

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===[[Team:Macquarie/Protocols/Making competent Cells|Making Competent Cells]]===
===[[Team:Macquarie/Protocols/Making competent Cells|Making Competent Cells]]===

Revision as of 03:42, 14 August 2012



Contents

Tuesday 7/8/12

(Click on headings to visit methods)

Making liquid media, plates & buffers

  • Liquid LB Media
  • SOC Solution
  • SOB Solution
  • LB Agar Plates

Ampicillin LB Agar Plates: 31 plates

Chloramphenicol LB Agar Plates: 33 Plates

Kanamyacin LB Agar Plates: 32 Plates

  • TB buffer.
  • TAE buffer.
  • EDTA buffer.


Designing Gibson Assembly Fragments

  • Haemoxygenase Gene
  • Deinococcus radiodurans Bacteriophytochrome Gene
  • Agrobacterium tumefaciens Bacteriophytochrome Gene

Tuesday 14/8/12

(Click on headings to visit methods)

Making Competent Cells

  • Choosing Biobricks
  • Making competent cells
  • Transformations

Outreach Planning

  • Open Day
  • Secondary Education Seminars