Team:UC Chile/Cyano/Labook/week9

From 2012.igem.org

(Difference between revisions)
 
Line 16: Line 16:
*As Gibson assembly is not working, we still need to try if pPMQAK1 works in synechocystis. We'll take out the toxic by standard assembly and transform Synechocystis afterwards.
*As Gibson assembly is not working, we still need to try if pPMQAK1 works in synechocystis. We'll take out the toxic by standard assembly and transform Synechocystis afterwards.
*Negative control of competent cells with pPMQAK1 was positive. Possible contamination.
*Negative control of competent cells with pPMQAK1 was positive. Possible contamination.
-
*Colonies in Gibson negative controls.
+
*There are colonies in Gibson negative controls.
 +
</font></p>
 +
<br>
 +
<p><font face="Calibri"><font size="4">Wednesday</font></font></p>
 +
<br>
 +
<p><font face="Calibri">
 +
*Gibson assembly for C1.1, C 1/2 and positive control.</font></p>
 +
<br>
 +
<p><font face="Calibri"><font size="4">Friday</font></font></p>
 +
<br>
 +
<p><font face = "Calibri">
 +
*Colony PCR for C1 and C1/2.
 +
*New competent cells prepared.
</font></p>
</font></p>

Latest revision as of 21:37, 13 August 2012

Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012


April 30 - May 6


Monday


  • Electrophoresis for constructs C1.1 and C1.2 assembled through Gibson. Wrong size.
  • PCR run of parts C1.1 and C1.2 for new Gibson.


Tuesday


  • KanR + double terminator has strange results. An analytic digestion will be done to check insertion.
  • As Gibson assembly is not working, we still need to try if pPMQAK1 works in synechocystis. We'll take out the toxic by standard assembly and transform Synechocystis afterwards.
  • Negative control of competent cells with pPMQAK1 was positive. Possible contamination.
  • There are colonies in Gibson negative controls.


Wednesday


  • Gibson assembly for C1.1, C 1/2 and positive control.


Friday


  • Colony PCR for C1 and C1/2.
  • New competent cells prepared.

Retrieved from "http://2012.igem.org/Team:UC_Chile/Cyano/Labook/week9"