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Team:KAIT Japan/Notebook
From 2012.igem.org
(Difference between revisions)
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During the creation<br> | During the creation<br> | ||
| - | + | <big>Creating parts of Tar methylation region.<big> | |
==8/9== | ==8/9== | ||
===Colony PCR=== | ===Colony PCR=== | ||
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*Template(E.coli DH5α) | *Template(E.coli DH5α) | ||
→'''Reflection:Took colony too many.You should take less colony.''' | →'''Reflection:Took colony too many.You should take less colony.''' | ||
| + | |||
---- | ---- | ||
==8/11== | ==8/11== | ||
Revision as of 02:53, 13 August 2012
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During the creation
Creating parts of Tar methylation region.
Contents |
8/9
Colony PCR
Reagent
- TaKaRa Ex Taq(5units/μL) 0.5μL
- 10×Ex Taq buffer 10μL
- dNTP Mixture(2.5Meach) 8μL
- Primer F(10μM) 4μL
- Primer R(10μM) 4μL
- Template(E.coli DH5α)
→Reflection:Took colony too many.You should take less colony.
8/11
The purified DNA1,2
- Electrophoresis
- Marker:pigment(buffer) 1μL,DNA molecule 2μL,TE buffer 3μL
- Sample:pigment(buffer) 1μL,sample 5μL
- Gel concentration:1.2%,Migration time:30minutes
- Storage
PCR Product 1,3,9
- Electrophoresis
- The gel check and cut
- DNA purification
- Confirmation of electrophoresis
- PCR
- 50cycle
- Storage
