"
Team:Amsterdam/project/diary/lab
From 2012.igem.org
| Line 3: | Line 3: | ||
{{Team:Amsterdam/Header}} | {{Team:Amsterdam/Header}} | ||
{{Team:Amsterdam/Sidebar1}} | {{Team:Amsterdam/Sidebar1}} | ||
| + | <html> | ||
<div id="main-content"> | <div id="main-content"> | ||
| - | <p></p> | + | <p> |
| + | <b><center><h1>Lab Journal</h1></center></b> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <b><center><h2>April</h2></center></b> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <u><center><h4>26/04/2012</h4></center></u> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. | ||
| + | We will do this by cloning a GFP into a pET28 vector using our transformation protocol. | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <u><center><h4>27/04/2012</h4></center></u> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix. | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <b><center><h2>May</h2></center></b> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <u><center><h4>07/05/2012</h4></center></u> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | Preparing O/N cultures which can be taken out of 37C in the weekend by a friend | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <u><center><h4>08/05/2012</h4></center></u> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | Our friend former igem member Yuri of 2011 will take out our O/N cultures. | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <u><center><h4>09/05/2012</h4></center></u> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | Yuri forgot... Start again. Note; rely on ourselves | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | <u><center><h4>10/05/2012</h4></center></u> | ||
| + | </p> | ||
| + | |||
| + | <p> | ||
| + | The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. | ||
| + | Gel: | ||
| + | Starting our first PCR. | ||
| + | </p> | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | |||
</div> | </div> | ||
| + | </html> | ||
{{Team:Amsterdam/Foot}} | {{Team:Amsterdam/Foot}} | ||
Latest revision as of 12:08, 9 August 2012
Lab Journal
April
26/04/2012
We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. We will do this by cloning a GFP into a pET28 vector using our transformation protocol.
27/04/2012
Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix.
May
07/05/2012
Preparing O/N cultures which can be taken out of 37C in the weekend by a friend
08/05/2012
Our friend former igem member Yuri of 2011 will take out our O/N cultures.
09/05/2012
Yuri forgot... Start again. Note; rely on ourselves
10/05/2012
The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. Gel: Starting our first PCR.
