From 2012.igem.org
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| | <h2>Tuesday, 7th August</h2> | | <h2>Tuesday, 7th August</h2> |
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| | Midi-prep pNHK60 (Tir1/GFP-aid), | | Midi-prep pNHK60 (Tir1/GFP-aid), |
| | Mini-prep pSB1C3 K515100 (IaaH+IaaM). | | Mini-prep pSB1C3 K515100 (IaaH+IaaM). |
Revision as of 14:13, 7 August 2012
Weeks:
| June |
July |
August |
September |
October |
November |
Week 9: 6th August - 12th August
Monday, 6th August
Reporter test
We had a lot of reporters BB but we didn't know if they're all working. We test them with a electrophoresis:
Two RFP, a GFP, a CFP and a YFP worked.
GFP BB
Digestion of GFP BB with EcoRI and PstI, gel extraction and DNA purification.
Goal: insertion in pCS2+ BB
Issue: Not enough DNA for ligation.
Mini-prep pCS2+-dendra2 and pCS2+-mcherry checked by gel.
inolation for midi-prep pNHK60 (Tir1/GFP-aid),
inoculation for mini-prep pSB1C3 K515100 (IaaH+IaaM).
Tuesday, 7th August
Midi-prep pNHK60 (Tir1/GFP-aid),
Mini-prep pSB1C3 K515100 (IaaH+IaaM).
Digestion pSB1C3 K515100 (IaaH+IaaM)with EcoRI and PstI then transformation in pCS2+ (amplified with PCR, digested with EcoRI and PstI then purified).
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