"
Team:Amsterdam/project/diary/lab
From 2012.igem.org
| Line 3: | Line 3: | ||
{{Team:Amsterdam/Header}} | {{Team:Amsterdam/Header}} | ||
{{Team:Amsterdam/Sidebar1}} | {{Team:Amsterdam/Sidebar1}} | ||
| + | <html> | ||
<div id="main-content"> | <div id="main-content"> | ||
<p> | <p> | ||
| Line 72: | Line 73: | ||
</div> | </div> | ||
| + | </html> | ||
{{Team:Amsterdam/Foot}} | {{Team:Amsterdam/Foot}} | ||
Revision as of 05:57, 7 August 2012
Lab Journal
April
26/04/2012
We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. We will do this by cloning a GFP into a pET28 vector using our transformation protocol.
27/04/2012
Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix.
May
07/05/2012
Preparing O/N cultures which can be taken out of 37C in the weekend by a friend
08/05/2012
Our friend former igem member Yuri of 2011 will take out our O/N cultures.
09/05/2012
Yuri forgot... Start again. Note; rely on ourselves
10/05/2012
The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. Gel: Starting our first PCR.
