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Team:Grenoble/Biology/Notebook/June/week 25
From 2012.igem.org
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<h1> Week 25: June 18<span class="exposant">th</span> to 24<span class="exposant">th</span> </h1> | <h1> Week 25: June 18<span class="exposant">th</span> to 24<span class="exposant">th</span> </h1> | ||
| - | After a meeting with our instructors, we decided to work on a second amplifier (cAMP-CRP system). We designed the genetic network for the second amplifier according to results we found in the literature | + | After a meeting with our instructors, we decided to work on a second amplifier (cAMP-CRP system). We designed the genetic network for the second amplifier according to results we found in the literature: <br/> |
| + | <br/> | ||
| + | <center><img src="https://static.igem.org/mediawiki/2012/d/d5/Network_cAMP-CRP.jpg" alt="pLAC_rsmY"/></center> | ||
<br/> | <br/> | ||
We looked for the biobricks involved in this network and we designed the primers for both amplifiers (RsmA-rsmY and cAMP-CRP systems) that we wanted to test in parallel.<br/> | We looked for the biobricks involved in this network and we designed the primers for both amplifiers (RsmA-rsmY and cAMP-CRP systems) that we wanted to test in parallel.<br/> | ||
Revision as of 16:12, 6 August 2012
Week 25: June 18th to 24th
After a meeting with our instructors, we decided to work on a second amplifier (cAMP-CRP system). We designed the genetic network for the second amplifier according to results we found in the literature:
We looked for the biobricks involved in this network and we designed the primers for both amplifiers (RsmA-rsmY and cAMP-CRP systems) that we wanted to test in parallel.
We looked for the plasmids on which we wanted to insert the selected biobricks in order to construct the amplifiers and to test them.
We searched in the literature to find a way to build a detection module.
