"
Team:Amsterdam/project/diary/lab
From 2012.igem.org
| Line 5: | Line 5: | ||
<div id="main-content"> | <div id="main-content"> | ||
<p> | <p> | ||
| - | <b><center>< | + | <b><center><h0>Lab Journal</h0></center></b> |
</p> | </p> | ||
Revision as of 10:13, 6 August 2012
Contents
26/04/2012
We arrive at the lab to get started! First items on the agenda are getting settled at the lab and acquiring our needed equipment. As a bonus we do a transformation need tot test the Gibson method we will be using throughout these months. We will do this by cloning a GFP into a pET28 vector using our transformation protocol.
27/04/2012
Succesfully transformed the plasmids. Ordered the primers for our gibson assembly and started to prepare things. Aliquoting our phusion and gisbon master mix.
07/05/2012
Preparing O/N cultures which can be taken out of 37C in the weekend by a friend
08/05/2012
Our friend former igem member Yuri of 2011 will take out our O/N cultures.
09/05/2012
Yuri forgot... Start again. Note; rely on ourselves
10/05/2012
The primers arrived. we reconstituded them and tried them on the plasmids after mini-prepping our O/N culture. Gel: Starting our first PCR.
