Team:UC Davis/Project/Catalyst

From 2012.igem.org

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We are looking to overproduce the LC-Cutinase gene in <i>E. Coli</i> so that we can further characterize its ability to degrade PET.
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Revision as of 22:44, 3 August 2012

Team:UC Davis - 2012.igem.org


Cutinase

The iGEM team at UC Davis is working to produce a catalyst capable of degrading the common plastic, PET. Further description to come.

Background
When looking for a way to degrade PET, we came across a paper that conducted a metagenomic analysis of leaf-branch compost, identified a cutinase homolog, and demonstrated its PET-degrading activity [1].

What we're doing
We are looking to overproduce the LC-Cutinase gene in E. Coli so that we can further characterize its ability to degrade PET.

References
[1] S. Sulaiman, S. Yamato, E. Kanaya, J. Kim, Y. Koga, K. Takano, S. Kanaya. "Isolation of a Novel Cutinase Homolog with Polyethylene Terephthalate-Degrading Activity from Leaf-Branch Compost by Using a Metagenomic Approach." Applied and Environment Microbiology, vol. 78 no. 5, pp. 1556-1562, March 2012.

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