Team:Carnegie Mellon

From 2012.igem.org

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<li class="toclevel-1"><a href="#Introduction:_Motivation"><span class="tocnumber">1</span> <span class="toctext">Introduction: Motivation</span></a></li>
<li class="toclevel-1"><a href="#Introduction:_Motivation"><span class="tocnumber">1</span> <span class="toctext">Introduction: Motivation</span></a></li>
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<li class="toclevel-1"><a href="#Primary_Objective:_A_single_sustainable_bacterial_solution"><span class="tocnumber">2</span> <span class="toctext">Primary Objective: A single sustainable bacterial solution</span></a></li>
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<li class="toclevel-1"><a href="#Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"><span class="tocnumber">2</span> <span class="toctext">Primary Objective: A single sustainable bacterial solution</span></a></li>
<li class="toclevel-1"><a href="#Secondary_Objective:_Solving_vitamin_A_deficiency_in_the_developing_world"><span class="tocnumber">3</span> <span class="toctext">Secondary Objective: Solving vitamin A deficiency in the developing world</span></a></li>
<li class="toclevel-1"><a href="#Secondary_Objective:_Solving_vitamin_A_deficiency_in_the_developing_world"><span class="tocnumber">3</span> <span class="toctext">Secondary Objective: Solving vitamin A deficiency in the developing world</span></a></li>
<li class="toclevel-1"><a href="#Further_Considerations"><span class="tocnumber">4</span> <span class="toctext">Further Considerations</span></a></li>
<li class="toclevel-1"><a href="#Further_Considerations"><span class="tocnumber">4</span> <span class="toctext">Further Considerations</span></a></li>
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<a name="Introduction:_Motivation"></a><h2> <span class="mw-headline"> Introduction: Motivation </span></h2>
<a name="Introduction:_Motivation"></a><h2> <span class="mw-headline"> Introduction: Motivation </span></h2>
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<ul><li> It has been predicted that by 2015 supplies of easy-to-access oil and natural gas will no longer keep up with demand. This prediction is based on the current rate of consumption, but this is expect to increase. The repercussions of this are already being felt on a global level with food prices rising.
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<ul><li> We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
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<ul><li> The burning of fossil fuels is also fuelling global warming. This is having negative effects on crop yields worldwide, with longer droughts occurring year on year, especially in Africa and Australia. The melting of the polar ice-caps which accompanies this will raise sea-levels, inundating currently arable land, reducing the land available for an ever enlarging world population.
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<ul><li> We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
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<ul><li> At present the major alternative to fossil fuel use for transport comes from ethanol fermented from starch and sugar in sugar cane and soy bean. These biofuel crops are being grown in areas previously used for food crops or in previously pristine natural environments, and thus are unsustainable.<br />
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<ul><li> We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.<br />
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All this is burdening the global economy and destroying lives, and worse is likely to come.<br />
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Place abstract/info here<br />
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<b>Wouldn't it be brilliant if we could do something to counter this trend?</b><br />
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<b>Motivation question?</b><br />
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This is what the Carnegie_Mellon 2012 iGEM team have been trying to do. - The rising cost of food coupled to the current unsustainability of human activity makes this the perfect time to contemplate the restructuring of global agriculture.<br />
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Humanistic implications go here<br />
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<a name="Primary_Objective:_A_single_sustainable_bacterial_solution"></a><h2> <span class="mw-headline"> Primary Objective: A single sustainable bacterial solution </span></h2>
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<a name="Primary_Objective:_A_Useful_BioBrick_for_Synthetic_Biologists"></a><h2> <span class="mw-headline"> Primary Objective: A single sustainable bacterial solution </span></h2>
<div class="thumb tright"><div class="thumbinner" style="width:152px;"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="image" title="A false-colour SEM of MicroMaize cells."><img alt="A false-colour SEM of MicroMaize cells." src="/wiki/images/thumb/0/08/Carnegie_Mellon-MicroMaize.jpg/150px-Carnegie_Mellon-MicroMaize.jpg" width="150" height="301" border="0" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>A false-colour SEM of MicroMaize cells.</div></div></div>
<div class="thumb tright"><div class="thumbinner" style="width:152px;"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="image" title="A false-colour SEM of MicroMaize cells."><img alt="A false-colour SEM of MicroMaize cells." src="/wiki/images/thumb/0/08/Carnegie_Mellon-MicroMaize.jpg/150px-Carnegie_Mellon-MicroMaize.jpg" width="150" height="301" border="0" class="thumbimage" /></a>  <div class="thumbcaption"><div class="magnify"><a href="/Image:Carnegie_Mellon-MicroMaize.jpg" class="internal" title="Enlarge"><img src="/wiki/skins/common/images/magnify-clip.png" width="15" height="11" alt="" /></a></div>A false-colour SEM of MicroMaize cells.</div></div></div>
<p>We have been investigating engineering bacteria to <b>produce starch from the cellulose in waste biomass</b> (that is agricultural waste, wood chippings, waste from paper production etc.). This starch could be:
<p>We have been investigating engineering bacteria to <b>produce starch from the cellulose in waste biomass</b> (that is agricultural waste, wood chippings, waste from paper production etc.). This starch could be:

Revision as of 18:53, 22 May 2012

Team:Carnegie_Mellon - 20012.igem.org

Team:Carnegie_Mellon

From 2012.igem.org

Contents

Introduction: Motivation

  • We seek to develop a BioBrick that will allow researchers in the field of synthetic biology to accurately measure translational efficiency, and transcriptional strength.
  • We believe that we can use Spinach as a biosensor to reflect these metrics in vivo, rather than in vitro, which has previously proven to be very costly and impractical.
  • We will characterize the relationship between genetic expression of Spinach (upstream), translational efficiency, and transcriptional strength.


Place abstract/info here

Motivation question?

Humanistic implications go here

Primary Objective: A single sustainable bacterial solution

A false-colour SEM of MicroMaize cells.
A false-colour SEM of MicroMaize cells.

We have been investigating engineering bacteria to produce starch from the cellulose in waste biomass (that is agricultural waste, wood chippings, waste from paper production etc.). This starch could be:

  1. sold to the biofuels industry for conversion to ethanol
  2. used as feed for livestock
  3. used as a starch supplement in the human diet if needed.

This will reduce our dependence on fossil fuels and free up agricultural land for the growth of food crops, ultimately putting less strain on ecosystems.

Secondary Objective: Solving vitamin A deficiency in the developing world

We have also been continuing the work of our 2007 team in engineering Escherichia coli to produce the vitamin A precursor β-carotene. Vitamin A is required for vision and a healthy immune system. 250,000-500,000 children in the developing world lose their vision each year, half of them dying within 12 months of this (WHO).

Further Considerations

In the pursuit of our project, as well as the biological aspects, we:

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