Team:Evry/Notebook/w8
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Revision as of 21:15, 2 August 2012
Weeks
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 |
Week 8: 30th July - 5th August
Monday, 30th July
Digestion - Ligation - Transformation:
Digestion of psc2+ and mRFP with EcoRI and PstI.Ligation and Transformation on T10.
Tuesday, 31th July
Inoculation:
Inoculation of pcs2+ (27.07) and repiquage of psc2+ with RFP (30.07)Inoculation of psB1C3 with GFP-AID TirI: Electrophoresis of 27/07 results: Failure of second PCR We restart the first step