Team:WashU/Week10

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Revision as of 00:20, 2 August 2012



Monday, July 30

Failed PCR in morning

Digestion of Z construct and tet-resistant plasmid with E and S. Run on gel

PCR using KlenTaq - worked

Transformed GC5 cells with Z-construct and U Plated cells to check tomorrow


Tuesday, July 21

Colony PCR on TOPO Ligation of Z construct to T plasmid PCR of Z and U constructs


Wednesday, August 1
Today we conducted a colony PCR of the TOPO cloning of ZCD with the fusion protein (without a his-tag). Colony PCR suggested that colony number 2 had the correct orientation of the insert (gel below). This colony will be grown up overnight and minipreped in the morning.
Thursday, August 2


Friday, August 3

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