Team:WashU/Protocols/Celllysis

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Revision as of 18:41, 26 July 2012


Cell Lysis and Protein Purification Prep

Buffer:

  1. The appropriate amount of tris(hydroxymethyl)aminomethane (Tris) was weighed to create a 50 mM solution. (6.057g/ liter of solution).
  2. Similarly, ethylenediaminetetraacetic acid (EDTA) was weighed out to create a 1 mM solution. (0.292g/ liter of solution).
  3. The two substances (Tris and EDTA) were dissolved in deionized water. (The solution had some difficulty dissolving and so was placed in a 37°C water bath for a few hours.)
  4. Now, the solution was pH-ed using 1 M HCl to a pH of 7.5. (NaOH may be added in the event of an overshoot.)


Lysis:

  1. The