"
Team:Cambridge/Protocols
From 2012.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
{{Template:Team:Cambridge/CAM_2012_TEMPLATE_HEAD_DIARY}} | {{Template:Team:Cambridge/CAM_2012_TEMPLATE_HEAD_DIARY}} | ||
| - | * [[Team:Cambridge/Protocols/PCRProtocol|<span style="color:# | + | * [[Team:Cambridge/Protocols/PCRProtocol|<span style="color:#000080">PCR using Phusion DNA polymerase</span>]] A method for amplifying a section of DNA. |
* [[Team:Cambridge/Protocols/PCRcolony|Colony PCR]] PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid. | * [[Team:Cambridge/Protocols/PCRcolony|Colony PCR]] PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid. | ||
* [[Team:Cambridge/Protocols/GelElectrophoresis|Gel Electrophoresis]] A technique for separating DNA strands of different lengths. | * [[Team:Cambridge/Protocols/GelElectrophoresis|Gel Electrophoresis]] A technique for separating DNA strands of different lengths. | ||
Revision as of 23:01, 24 July 2012
- PCR using Phusion DNA polymerase A method for amplifying a section of DNA.
- Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
- Gel Electrophoresis A technique for separating DNA strands of different lengths.
- Gel Extraction of DNA A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis.
- Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.
- Transformation of Escherichia coli A method for transforming competent E.coli with DNA
- Transformation of Bacillus subtilis A technique used to introduce foreign DNA into competent Bacillus cells.
- MiniPrep A method used to extract DNA from bacterial cells.
- Restriction Enzyme Digest A method for creating a restriction map of a plasmid.
- Protein analysis by SDS PAGE A method used to separate polypeptides of different lengths.
- Preparation of LB Agar Plates A method used to prepare agar plate to culture common bacteria.
N.B. Information in purple is subject to change through optimisation over the course of our project.
