Team:Fudan D

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#menu p, #menu h2, #menu h3, #menu ul li {
+
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font-family:Arial, Helvetica, sans-serif;
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+
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+
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.strong {
+
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-
font-weight:bold;
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}
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.italic {
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-
font-style:italic;
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-
}
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.imgshadow { /* Better style on light background */
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-
background:#FFFFFF;
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-
padding:4px;
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-
 
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+
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/* Rounded Corners */
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    border-radius: 5px;
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/* Shadow */
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-
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-moz-box-shadow:inset 0 0 3px #000000;
+
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box-shadow:inset 0 0 3px #000000;
+
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-
#menu li ul {
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-
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-
padding:0;
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+
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}
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-
#menu li ul li {
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-
font-size:12px;
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float:none;
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text-align:left;
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width:130px;
+
-
 
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-
}
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-
#menu li ul li:hover {
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background:none;
+
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border:none;
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-
padding:0;
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-
 
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margin:0;
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}
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-
 
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-
 
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-
 
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-
#menu li .greybox li {
+
-
 
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-
background:#F4F4F4;
+
-
 
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-
border:1px solid #bbbbbb;
+
-
 
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-
margin:0px 0px 4px 0px;
+
-
 
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-
padding:4px 6px 4px 6px;
+
-
 
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-
width:116px;
+
-
 
+
-
 
+
-
 
+
-
/* Rounded Corners */
+
-
 
+
-
-moz-border-radius: 5px;
+
-
 
+
-
    -webkit-border-radius: 5px;
+
-
 
+
-
    -khtml-border-radius: 5px;
+
-
 
+
-
    border-radius: 5px;
+
-
 
+
-
}
+
-
 
+
-
#menu li .greybox li:hover {
+
-
 
+
-
background:#ffffff;
+
-
 
+
-
border:1px solid #aaaaaa;
+
-
 
+
-
padding:4px 6px 4px 6px;
+
-
 
+
-
margin:0px 0px 4px 0px;
+
-
 
+
-
}
+
-
 
+
-
 
+
-
 
+
-
div#spanner {
+
-
 
+
-
    position:absolute;
+
-
 
+
-
    top:160px;
+
-
 
+
-
    left:40px;
+
-
 
+
-
    width:920px;
+
-
 
+
-
    height:334px;
+
-
 
+
-
    background:url(https://static.igem.org/mediawiki/2011/8/82/YaleFg.png) no-repeat;
+
-
 
+
-
}
+
-
 
+
-
div#text{
+
-
 
+
-
    position:absolute;
+
-
 
+
-
    top:160px;
+
-
 
+
-
    left:40px;
+
-
 
+
-
    width:920px;
+
-
 
+
-
    height:334px;
+
-
 
+
-
}
+
-
 
+
-
div#container {
+
-
        /* Rounded Corners */ 
+
-
 
+
-
    -moz-border-radius: 10px; 
+
-
    -webkit-border-radius: 10px; 
+
-
    border-radius: 10px; 
+
-
 
+
-
    /* Background color and gradients */ 
+
-
 
+
-
    background: #014464; 
+
-
    background: -moz-linear-gradient(top, #0272a7, #013953); 
+
-
    background: -webkit-gradient(linear, 0% 0%, 0% 100%, from(#0272a7), to
+
-
 
+
-
(#013953)); 
+
-
 
+
-
    /* Borders */ 
+
-
 
+
-
    border: 1px solid #002232; 
+
-
 
+
-
    -moz-box-shadow:inset 0px 0px 1px #edf9ff; 
+
-
    -webkit-box-shadow:inset 0px 0px 1px #edf9ff; 
+
-
    box-shadow:inset 0px 0px 1px #edf9ff;
+
-
}
+
-
 
+
-
 
+
-
    div#spanner p#abstract {
+
-
 
+
-
        position:absolute;
+
-
 
+
-
        margin-left:420px;
+
-
 
+
-
        margin-top:35px;
+
-
 
+
-
        color:#ffffff;
+
-
        font-size:13px;
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
div#content {
+
-
 
+
-
    padding-top:450px;
+
-
 
+
-
    width:960px;
+
-
 
+
-
    padding-bottom:0px;
+
-
 
+
-
}
+
-
 
+
-
 
+
-
 
+
-
    div#content p {
+
-
 
+
-
        font-size:15px;
+
-
 
+
-
        margin-left:20px;
+
-
 
+
-
        margin-top:60px;
+
-
 
+
-
        margin-right:20px;
+
-
 
+
-
        text-align:justify;
+
-
 
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
    div#content div.col1 {
+
-
 
+
-
        float:left;
+
-
 
+
-
        background:url(https://static.igem.org/mediawiki/2011/e/eb/YaleCol1.png) no-repeat;
+
-
 
+
-
        margin:0px;
+
-
 
+
-
        padding:0px;
+
-
 
+
-
        width:320px;
+
-
 
+
-
        height:200px;
+
-
 
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
    div#content div.col2 {
+
-
 
+
-
        float:left;
+
-
 
+
-
        background:url(https://static.igem.org/mediawiki/2011/8/87/YaleCol2.png) no-repeat;
+
-
 
+
-
        margin:0px;
+
-
 
+
-
        padding:0px;
+
-
 
+
-
        width:320px;
+
-
 
+
-
        height:200px;
+
-
 
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
    div#content div.col3 {
+
-
 
+
-
        float:left;
+
-
 
+
-
        background:url(https://static.igem.org/mediawiki/2011/7/7d/YaleCol3.png) no-repeat;
+
-
 
+
-
        margin:0px;
+
-
 
+
-
        padding:0px;
+
-
 
+
-
        width:320px;
+
-
 
+
-
        height:200px;
+
-
 
+
-
    }
+
-
 
+
-
 
+
-
 
+
-
#center {
+
-
    text-align:center;
+
-
    padding-top:15px;
+
-
    padding-bottom:50px;
+
-
}
+
-
 
+
-
/* End CSS */
+
-
 
+
-
</style>
+
-
 
+
-
<!------------------- End CSS ------------------->
+
-
 
+
-
</head>
+
-
 
+
-
<!------------------- Begin Body ------------------->
+
-
<div id="page-wrap">
+
-
    <div id="outside-container">
+
-
        <div id="logo">
+
-
            <a href="https://2011.igem.org/Team:Yale"><img src="https://static.igem.org/mediawiki/2011/8/83/Igemyale.png" /></a>
+
-
        </div>
+
-
        <ul id="menu">
+
-
 
+
-
    <li><a href="https://2011.igem.org/Team:Yale">Home</a>
+
-
 
+
-
    <li><a href="https://2011.igem.org/Team:Yale/Project" class="drop">Project</a>
+
-
        <div class="dropdown_1column">
+
-
 
+
-
                        <div class="col_1">     
+
-
 
+
-
                            <ul class="simple">
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Project">Overview</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Project/Introduction">Introduction</a></li>
+
-
<li><a href="https://2011.igem.org/Team:Yale/Parts">BioBricks</a></li>
+
-
<li><a href="https://2011.igem.org/Team:Yale/Protein">Proteins</a></li>
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Project/Assays">Functional Assays</a></li>
+
-
<li><a href="https://2011.igem.org/Team:Yale/Project/Crystallography">Crystallography</a></li>
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Project/MAGE">MAGE</a></li>
+
-
<li><a href="https://2011.igem.org/Team:Yale/Project/References">References</a></li>
+
-
                               
+
-
 
+
-
 
+
-
 
+
-
                            </ul>   
+
-
 
+
-
                        </div>               
+
-
 
+
-
            </div>
+
-
            </li>
+
-
 
+
-
    <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week1" class="drop">Notebook</a>
+
-
        <div class="dropdown_1column">
+
-
 
+
-
                        <div class="col_1">     
+
-
 
+
-
                            <ul class="simple">
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Protocols">Protocols</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week1">Week 1</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week2">Week 2</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week3">Week 3</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week4">Week 4</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week5">See more weeks...</a></li>
+
-
 
+
-
                            </ul>   
+
-
 
+
-
                        </div>               
+
-
 
+
-
            </div>
+
-
        </li>
+
-
 
+
-
    <li><a href="https://2011.igem.org/Team:Yale/Safety">Safety</a></li>
+
-
 
+
-
    <li><a href="https://2011.igem.org/Team:Yale/Team" class="drop">About</a>
+
-
        <div class="dropdown_1column">
+
-
 
+
-
                        <div class="col_1">     
+
-
 
+
-
                            <ul class="simple">
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Team">Our team</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Team/Advisors">Our advisors</a></li>
+
-
 
+
-
                                <li><a href="https://2011.igem.org/Team:Yale/Team/Acknowledgements">Acknowledgements</a></li>
+
-
 
+
-
                            </ul>   
+
-
 
+
-
                        </div>               
+
-
 
+
-
            </div>
+
-
        </li>
+
-
 
+
-
        </ul>
+
-
    </div>
+
</div>
</div>
-
 
+
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
 +
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
</div>
</div>
-
</body>
+
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
-
 
+
You <strong>MUST</strong> have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.
-
</p><p><br />
+
-
 
+
-
  <div id="spanner">
+
-
      <p id="abstract">
+
-
<b>Nature’s Antifreeze: Microbial Expression and Characterization of a Novel Insect Antifreeze Protein for De-icing Solutions</b><br /><br />
+
-
            Antifreeze proteins have applications in cryopreservation of food, cells, and organs, as well as in cryosurgery and agriculture. The purpose of this study was to express, purify, characterize, and optimize a novel, hyperactive antifreeze protein recently isolated from the Siberian beetle, Rhagium inquisitor (RiAFP). Large scale (150mg/L), stable production of RiAFP and a RiAFP-GFP fusion protein was achieved in E. coli. Proteins were purified by Ni-NTA affinity chromatography. E. coli expressing RiAFP exhibited increased survival post-freezing. RiAFP inhibited ice recrystallization in a dose-dependent manner. RiAFP also improved tissue morphology of rat livers post-freezing. Preliminary results indicate that RiAFP may have a cryoprotective effect in C. elegans. To optimize the activity of the hypothesized RiAFP binding site, we used directed evolution through multiplex automated genome engineering and are currently screening for mutants with enhanced properties. Finally, to better understand the structure-function relationship, we have generated promising crystals of RiAFP for x-ray crystallography and are now optimizing crystallization conditions.
+
-
      </p>           
+
-
  </div>
+
-
        <div id="contents">
+
-
            <div class="col1">
+
-
                <p>We demonstrate that the <i>Rhagium inquisitor</i> antifreeze protein possesses great potential for applications requiring freeze resistance or the control of ice growth and morphology.</p>
+
-
                <div id="center"><a href="https://2011.igem.org/Team:Yale/Project"><img src="https://static.igem.org/mediawiki/2011/2/21/YaleBut1.png" /></a></div>
+
-
 
+
-
            </div>
+
-
            <div class="col2">
+
-
                <p>We expressed, purified, characterized and have begun optimizing the novel, hyperactive <i>Rhagium inquisitor</i> antifreeze protein using a variety of synthetic and molecular biology techniques.</p>
+
-
                <div id="center"><a href="https://2011.igem.org/Team:Yale/Protocols"><img src="https://static.igem.org/mediawiki/2011/a/ac/YaleBut2.png" /></a></div>
+
-
 
+
-
            </div>
+
-
            <div class="col3">
+
-
                <p>A second year entrant to the iGEM competition, the Yale iGEM team is comprised of students from a wide range of backgrounds connected by a common passion for synthetic biology. </p>
+
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Retrieved from "<a href="https://2011.igem.org/Team:Yale">https://2011.igem.org/Team:Yale</a>"</div>
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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''Tell us more about your project.  Give us background.  Use this as the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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|align="center"|[[Team:Fudan_D | Team Fudan_D]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Fudan_D/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Fudan_D Official Team Profile]
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Revision as of 02:59, 22 July 2012


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have all of the pages listed in the menu below with the names specified. PLEASE keep all of your pages within your teams namespace.



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Fudan D logo.png

Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:Fudan D team.png
Your team picture
Team Fudan_D


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions
Retrieved from "http://2012.igem.org/Team:Fudan_D"