Team:WashU/Week8
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- | This morning, we miniprepped a culture of PC42, our ligation of plasmid PSL2131 and construct CS42S. Then, we ran a gel of double and single digests of PC42, cut with X and P and just P, along with an uncut PC42 as a control. (2x refers to ligation cut with both X and P, 1x indicates ligation cut with P, and 0x signifies uncut PC42) | + | This morning, we miniprepped a culture of PC42, our ligation of plasmid PSL2131 and construct CS42S. Then, we ran a gel of double and single digests of PC42, cut with X and P and just P, along with an uncut PC42 as a control. (2x refers to ligation cut with both X and P, 1x indicates ligation cut with P, and 0x signifies uncut PC42) The band sizes resulting from the digestion match the sizes of the plasmid and construct, indicating that the ligation worked. |
<div align="center">https://lh5.googleusercontent.com/--ulNrdD-1xs/UAhk8NfQmeI/AAAAAAAAAV0/BVhDt7rnPAY/s800/pc42digest%25207-18.jpg</div><br> | <div align="center">https://lh5.googleusercontent.com/--ulNrdD-1xs/UAhk8NfQmeI/AAAAAAAAAV0/BVhDt7rnPAY/s800/pc42digest%25207-18.jpg</div><br> | ||
Revision as of 20:19, 19 July 2012