From 2012.igem.org

Revision as of 19:42, 19 July 2012

Tasks

• Make cloramphenicol stock. Check.
• Make glycerol stocks of important parts. Discard old plates. Check.
• Perform CFU-assay of lysis induced cells. Check. Redo?
• Combine LuxR-HSL and lysis device.
• Combine pBAD and GFP

• Research lactate production in E. coli
• Write more for the book chapter
• Research QuikChange

Useful links

http://www.nanodrop.com/Support.aspx?Type=Maintenance&Cat=NanoDrop%201000

http://www.troemner.com/pdf/PipetteStandardsHandbook.pdf'

http://www.pipette.com/Support/GuideToPipetting.pdf

https://2008.igem.org/Team:EPF-Lausanne/2-step_PCR

https://2009.igem.org/Team:UNIPV-Pavia/Parts_Characterization#Microplate_reader_experiments

[http://www.jbioleng.org/content/5/1/17 Amplified insert assembly: an optimized approach to standard assembly of BioBrickTM genetic circuits]

Lactate genes and promoters:

[http://www.biomedcentral.com/1471-2180/10/321#B24 Quinone-dependent D-lactate dehydrogenase Dld (Cg1027) is essential for growth of Corynebacterium glutamicum on D-lactate]

[http://aem.asm.org/content/71/10/5920.full Characterization of a Corynebacterium glutamicum Lactate Utilization Operon Induced during Temperature-Triggered Glutamate Production†]

[http://www.genome.jp/dbget-bin/www_bget?cgb:cg1027 KEGG: cg1027]

[http://jb.asm.org/content/191/13/4251.abstract The ldhA Gene, Encoding Fermentative l-Lactate Dehydrogenase of Corynebacterium glutamicum, Is under the Control of Positive Feedback Regulation Mediated by LldR

[http://mic.sgmjournals.org/content/142/5/1297 Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif]

[http://www.sciencedirect.com/science/article/pii/S016816560300155X Promoters of Corynebacterium glutamicum]

DNA oligo and gene synthesis

http://www.invitrogen.com/site/us/en/home/Products-and-Services/Product-Types/Primers-Oligos-Nucleotides/invitrogen-custom-dna-oligos/Technical-Resources-for-Oligonucleotides/DNA-Oligo-FAQ.html#1

PCR

[http://irc.igd.cornell.edu/Protocols/PCR_principles.htm PCR Principles and Practices]

[http://www.neb.com/nebecomm/products/protocol54.asp New England Biolabs: PCR Guidelines]

http://www.uq.edu.au/vdu/PDU_PCRPrimers.htm

[http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html Premier biosoft: PCR primer design guidelines]

http://www.cybertory.org/exercises/primerDesign/index.html

http://www.mcdb.lsa.umich.edu/labs/maddock/protocols/PCR/general_pcr_protocol.html

[http://www.miraibio.com/blog/2008/03/10-tips-for-designing-pcr-primers-that-work/ 10 tips for designing PCR primers that work]

[http://www.ehow.com/how_7242346_make-oligonucleotide-primers-pcr.html How to make oligonucleotide primers for PCR]

Software

http://molbiol-tools.ca/molecular_biology_freeware.htm

quorum sensing

[http://www.annualreviews.org/doi/pdf/10.1146/annurev.micro.55.1.165 Quorum sensing in bacteria - Annu. Rev. Microbiol. 2001. 55:165–99]

[http://www.ncbi.nlm.nih.gov/pubmed/16487323 N-acyl-L-homoserine lactone signal interception by Escherichia coli.]

Other

[http://www.bion.no/2011/11/syntetisk-biologi/ Syntetisk biologi: Genmodifisert bakterie drep annan bakterie]

http://en.wikipedia.org/wiki/DNA_ligase