"
Team:UC Chile/Cyano/Notepad/week20
From 2012.igem.org
| Line 66: | Line 66: | ||
Later on we checked yesterday's plates and all of them had colonies (yay!!), although the ones with newer ligation buffer had better yield (plates 1 and 5). The previous low transformation efficiencies might have been because of this. We culture colonies from plates 1 and 5 in bacterial tubes for further extraction. | Later on we checked yesterday's plates and all of them had colonies (yay!!), although the ones with newer ligation buffer had better yield (plates 1 and 5). The previous low transformation efficiencies might have been because of this. We culture colonies from plates 1 and 5 in bacterial tubes for further extraction. | ||
| + | |||
| + | Parts for our argentinian advisor are on their way to Cambridge :) hope they make it through customs! | ||
Revision as of 23:24, 17 July 2012
Monday: Today is suppose to be a holiday, I'm not really sure why, but the team is still working. The building seems abandoned and we are the only ones in here muajuajuajuajua (evil laughter).
About our work in progress: we have our second arduino project. It is a photoresistor able to measure the intensity of light inputs. The arduino program is linked to a c# aplication so we can see a graph of the data in real time. We have only tried it with LEDs so far and the system proved to be sensible enough. The next step is to measure bacterial biolumniscence. We hope to do so tomorrow. We transformed e. coli with lux brick (at race speed) in order to have a really nice shining flask and check if our arduino system can sense bacterial produced light properly.
Meanwhile in the wetlab... We built the following parts trough a ligation protocol. Then we transformed and plated e.coli.
1. RS1 + KanR + psb1C3 . Plate resistance: chloramphenicol and kanamycin.
2. RS1 + KanR. Plate resistance: chloramphenicol.
3. RS1+ KanR. Plate resistance: chloramphenicol and kanamycin.
4. B0014 + RS2 + psb1C3. Plate resistance: chloramphenicol.
5. B0014 + RS2. Plate resistance: kanamycin.
Also, we did an electrophoresis run of LuxCDEG PCR product. Nothing showed up u.u
PSB1C3 was purified for further use.
Finally, we prepared some parts to send to our international advisor (yeah, the Argentinian) so he can try to assemble them by the Gibson technique.
SIMON LEFT US TODAY :_( He'll be spending the next three (yes, THREE!) weeks in San Francisco. He couldn't help but trade us for sunny californian beaches...
Tuesday: We had a videoconference with Copenhagen and Valencia teams. As we are working in very similiar projects we decided to establish a collaborative alliance. Eventually, we will share our constructs to compare their performance in the different systems. Go teams go!!!
Later on we checked yesterday's plates and all of them had colonies (yay!!), although the ones with newer ligation buffer had better yield (plates 1 and 5). The previous low transformation efficiencies might have been because of this. We culture colonies from plates 1 and 5 in bacterial tubes for further extraction.
Parts for our argentinian advisor are on their way to Cambridge :) hope they make it through customs!
