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Team:Caltech/Notebook/Degradation
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| - | <font size="+2"><a name=" | + | <font size="+2"><a name="6_25_29_12">June 25 - 29, 2012</a></font> |
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<br> Sent out requests for bacterial strains and plasmids based on spreadsheet | <br> Sent out requests for bacterial strains and plasmids based on spreadsheet | ||
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<br> Emailed Dr. Jared Leadbetter asking if he has strains + protocol for lignocellulose plates | <br> Emailed Dr. Jared Leadbetter asking if he has strains + protocol for lignocellulose plates | ||
<br> Made carb. R plates | <br> Made carb. R plates | ||
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<br> Made minimal media agar plates, bottom layer of overlay plates | <br> Made minimal media agar plates, bottom layer of overlay plates | ||
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<br> Met with Professor Leadbetter to discuss difficulties with creating enrichment plates and finding bacterial samples | <br> Met with Professor Leadbetter to discuss difficulties with creating enrichment plates and finding bacterial samples | ||
<br> Will be following up on some of his suggestions | <br> Will be following up on some of his suggestions | ||
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<br> Notes from Meeting with Professor Jared Leadbetter from June 28th | <br> Notes from Meeting with Professor Jared Leadbetter from June 28th | ||
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| - | <br>Overlay Plate Options | + | <br> Overlay Plate Options |
| - | <br>- Top layer with minimal media, although either minimal media or just water + agarose should work | + | <br> - Top layer with minimal media, although either minimal media or just water + agarose should work |
| - | <br>- Embed bacteria into top layer mix | + | <br> - Embed bacteria into top layer mix |
| - | <br>- Use filter paper made of chosen carbon sources | + | <br> - Use filter paper made of chosen carbon sources |
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| - | <br>Bacterial Strains | + | <br> Bacterial Strains |
| - | <br>- Ponds around campus | + | <br> - Ponds around campus |
| - | <br>- Beach | + | <br> - Beach |
| - | <br>- Teredinibacter turnerae gen: helps degrade wood in shipworms (http://ijs.sgmjournals.org/content/52/6/2261.abstract) | + | <br> - Teredinibacter turnerae gen: helps degrade wood in shipworms (http://ijs.sgmjournals.org/content/52/6/2261.abstract) |
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| - | <br>May also choose a different host organism for transformation | + | <br> May also choose a different host organism for transformation |
| - | <br>- Instead of using E. coli can try using yeast (simplifies problem to only of degradation, and not also ethanol synthesis) | + | <br> - Instead of using E. coli can try using yeast (simplifies problem to only of degradation, and not also ethanol synthesis) |
| - | <br>- Can also try bacteria found by Aztecs to make alcohol (Zymomonas mobilis) | + | <br> - Can also try bacteria found by Aztecs to make alcohol (Zymomonas mobilis) |
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<br> Emailed for samples of nitrocellulose, latex, and teflon membranes. | <br> Emailed for samples of nitrocellulose, latex, and teflon membranes. | ||
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| - | <font size="+2"><a name=" | + | <font size="+2"><a name="7_2_6_12">July 2-6, 2012</a></font> |
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<br> Researched zymomonas mobilis, narrowed down focus to strain ZM4 | <br> Researched zymomonas mobilis, narrowed down focus to strain ZM4 | ||
<br> Gathered more information on protocols specific to that strain. | <br> Gathered more information on protocols specific to that strain. | ||
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<br> Looked up additional information about potential vectors to use to transform z. mobilis | <br> Looked up additional information about potential vectors to use to transform z. mobilis | ||
| - | <br> Requested p42-0119 plasmid for z. mobilis from | + | <br> Requested p42-0119 plasmid for expression z. mobilis from the Oak Ridge National Laboratory |
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<br> Made 20% glucose solution stock and RM plates for Z. mobilis | <br> Made 20% glucose solution stock and RM plates for Z. mobilis | ||
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| - | <font size="+2"><a name=" | + | <font size="+2"><a name="7_9_13_12">July 9-13, 2012</a></font> |
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<br> Ordered strains from the ATCC. | <br> Ordered strains from the ATCC. | ||
<br> Discussed Biolog plates idea | <br> Discussed Biolog plates idea | ||
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| - | < | + | <br> Research z mobilis degradation 5 and 6 carbon sugars |
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| - | + | <br> Researched for genetic sequences for sugar degradation enzymes. | |
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| - | <br> | + | <br> Sent in request for oligos sequences for alpha-gluctosidase gene (can degrade maltose) |
| + | <br> Made tetracycline plates | ||
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Degradation#Calendar">Back to Degradation Notebook Calendar</a> | <a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Degradation#Calendar">Back to Degradation Notebook Calendar</a> | ||
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| - | <br> | + | <font size="+2"><a name="7_16_20_12">July 16-20, 2012</a></font> |
| + | <br> Electroporated pSB1T3 into competent E coli cells, incubated in LB for an hour, and streaked transformants onto tetra plates | ||
| + | <br> Streaked DH5alpha E. coli strain with plasmid pMQ30 onto a gentamicin 20 ug/mL plate | ||
| + | <br> Streaked Z. mobilis ZM4 from ATCC onto a RM plate and liquid culture. | ||
| + | <br> Streaked out DKN79 (DH5alpha + pLAFR5) onto a tetracycline plate | ||
| + | <br> Streaked out DKN1005 (BW20427 [WM3064] + pLAFR5) onto a tetracycline plate | ||
| + | <br> Made LB media | ||
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<a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Degradation#Calendar">Back to Degradation Notebook Calendar</a> | <a a style="float:right;" href="https://2012.igem.org/Team:Caltech/Notebook/Degradation#Calendar">Back to Degradation Notebook Calendar</a> | ||
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Revision as of 04:01, 17 July 2012
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Degradation Notebook
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