Team:Caltech/Notebook/Degradation

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<br> Created a spreadsheet detailing various possible bacterial strains and plasmids to be used for complex polymer degradation
<br> Created a spreadsheet detailing various possible bacterial strains and plasmids to be used for complex polymer degradation
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<br> Sent out requests for bacterial strains and plasmids based on spreadsheet
<br> Sent out requests for bacterial strains and plasmids based on spreadsheet
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<font size="+2"><a name="6_26_12">June 26, 2012</a></font>
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<br> Emailed Dr. Jared Leadbetter asking if he has strains + protocol for lignocellulose plates
<br> Emailed Dr. Jared Leadbetter asking if he has strains + protocol for lignocellulose plates
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<br> Made minimal media agar plates, bottom layer of overlay plates
<br> Made minimal media agar plates, bottom layer of overlay plates
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<br> Met with Professor Leadbetter to discuss difficulties with creating enrichment plates and finding bacterial samples
<br> Met with Professor Leadbetter to discuss difficulties with creating enrichment plates and finding bacterial samples
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<br> Emailed for samples of nitrocellulose, latex, and teflon membranes.
<br> Emailed for samples of nitrocellulose, latex, and teflon membranes.
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<br> Researched zymomonas mobilis, narrowed down focus to strain ZM4
<br> Researched zymomonas mobilis, narrowed down focus to strain ZM4
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<br> Looked up additional information about potential vectors to use to transform z. mobilis
<br> Looked up additional information about potential vectors to use to transform z. mobilis
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<br> Made 20% glucose solution stock and RM plates for Z. mobilis
<br> Made 20% glucose solution stock and RM plates for Z. mobilis
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<br> Ordered strains from the ATCC.
<br> Ordered strains from the ATCC.
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<br> Research z mobilis degradation sugar targets
<br> Research z mobilis degradation sugar targets
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<br> Researched for genetic sequences for sugar degradation enzymes.
<br> Researched for genetic sequences for sugar degradation enzymes.

Revision as of 19:58, 16 July 2012




Degradation Notebook Calendar

June 2012
Sun Mon Tue Wed Thu Fri Sat
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
July 2012
Sun Mon Tue Wed Thu Fri Sat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
August 2012
Sun Mon Tue Wed Thu Fri Sat
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31


Degradation Notebook


June 22, 2012

Created a spreadsheet detailing various possible bacterial strains and plasmids to be used for complex polymer degradation
Back to Degradation Notebook Calendar
June 25, 2012

Sent out requests for bacterial strains and plasmids based on spreadsheet
Back to Degradation Notebook Calendar
June 26, 2012

Emailed Dr. Jared Leadbetter asking if he has strains + protocol for lignocellulose plates
Made carb. R plates
Back to Degradation Notebook Calendar
June 27, 2012

Made minimal media agar plates, bottom layer of overlay plates
Back to Degradation Notebook Calendar
June 28, 2012

Met with Professor Leadbetter to discuss difficulties with creating enrichment plates and finding bacterial samples
Will be following up on some of his suggestions

Notes from Meeting with Professor Jared Leadbetter from June 28th

Overlay Plate Options
- Top layer with minimal media, although either minimal media or just water + agarose should work
- Embed bacteria into top layer mix
- Use filter paper made of chosen carbon sources

Bacterial Strains
- Ponds around campus
- Beach
- Teredinibacter turnerae gen: helps degrade wood in shipworms (http://ijs.sgmjournals.org/content/52/6/2261.abstract)

May also choose a different host organism for transformation
- Instead of using E. coli can try using yeast (simplifies problem to only of degradation, and not also ethanol synthesis)
- Can also try bacteria found by Aztecs to make alcohol (Zymomonas mobilis)
Back to Degradation Notebook Calendar
June 29, 2012

Emailed for samples of nitrocellulose, latex, and teflon membranes.
Researched Z. mobilis.
Back to Degradation Notebook Calendar
July 2, 2012

Researched zymomonas mobilis, narrowed down focus to strain ZM4
Gathered more information on protocols specific to that strain.
Back to Degradation Notebook Calendar
July 3, 2012

Looked up additional information about potential vectors to use to transform z. mobilis
Requested p42-0119 plasmid for z. mobilis from another lab
Back to Degradation Notebook Calendar
July 5, 2012

Made 20% glucose solution stock and RM plates for Z. mobilis
Back to Degradation Notebook Calendar
July 9, 2012

Ordered strains from the ATCC.
Discussed Biolog plates idea
Back to Degradation Notebook Calendar
July 10, 2012

Research z mobilis degradation sugar targets
Back to Degradation Notebook Calendar
July 11, 2012

Researched for genetic sequences for sugar degradation enzymes.
Back to Degradation Notebook Calendar

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