"
Team:Cambridge/Protocols
From 2012.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
| + | {| style="color:#1b2c8a;background-color:#3ae2e8;" cellpadding="3" cellspacing="0" border="1" bordercolor="#fff" width="62%" align="center" | ||
| + | !align="center"|[[Team:Cambridge|Home]] | ||
| + | !align="center"|[[Team:Cambridge/Team|Team]] | ||
| + | !align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Cambridge Official Team Profile] | ||
| + | !align="center"|[[Team:Cambridge/Project|Project]] | ||
| + | !align="center"|[[Team:Cambridge/Parts|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Cambridge/Modeling|Modeling]] | ||
| + | !align="center"|[[Team:Cambridge/Notebook|Notebook]] | ||
| + | !align="center"|[[Team:Cambridge/Safety|Safety]] | ||
| + | !align="center"|[[Team:Cambridge/Attributions|Attributions]] | ||
| + | !align="center"|[[Team:Cambridge/Sponsors|Sponsors]] | ||
| + | |} | ||
| + | |||
| + | |||
* [[Team:Cambridge/Protocols/PCRProtocol|PCR using Phusion DNA polymerase]] A method for amplifying a section of DNA. | * [[Team:Cambridge/Protocols/PCRProtocol|PCR using Phusion DNA polymerase]] A method for amplifying a section of DNA. | ||
* [[Team:Cambridge/Protocols/PCRcolony|Colony PCR]] PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid. | * [[Team:Cambridge/Protocols/PCRcolony|Colony PCR]] PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid. | ||
Revision as of 13:00, 16 July 2012
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Notebook | Safety | Attributions | Sponsors |
|---|
- PCR using Phusion DNA polymerase A method for amplifying a section of DNA.
- Colony PCR PCR with cells as a template. Useful for checking the length of an insert in an introduced plasmid.
- Gel Electrophoresis A technique for separating DNA strands of different lengths.
- Gel Extraction of DNA A technique used to isolate a desired fragment of intact DNA from an agarose gel following agarose gel electrophoresis.
- Gibson Assembly A technique for ligating multiple DNA fragments in one step, compatible with standard assembly.
- Transformation of Bacillus subtilis A technique used to introduce foreign DNA into competent Bacillus cells.
- MiniPrep A method used to extract DNA from bacterial cells.
- Restriction Enzyme Digest A method for creating a restriction map of a plasmid.
- Protein analysis by SDS PAGE A method used to separate polypeptides of different lengths.
- Preparation of LB Agar Plates A method used to prepare agar plate to culture common bacteria.
N.B. Information in purple is subject to change through optimisation over the course of our project.
