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Revision as of 16:11, 14 July 2012

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Contents 1 Monday 2 Tuesday 3 Wednesday 4 Thursday 5 Friday

Monday

Making bacillus competent

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• Bacillus salts *10 made up and autoclaved.

• Medium A base *10 made up (glucose will be added tomorrow)

• bacillus strain 168 streaked out and grown on plate overnight.

Tuesday

Making bacillus competent

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• Filtered glucose added to sterile Medium A base.

• Sterile aliquots of salts and medium apportioned and put in fridge (10*50ml each)

Wednesday

Making bacillus competent

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Thursday

Test transformation of frozen bacillus stocks

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• Two samples of bacillus defrosted from different batches.

Friday

Transformation of bacillus and e.coli with lux genes from 2010

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Testing of the spectral properties of filters

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• Blue , Green and Red filters were tested in different wavelengths (400 nm - 600 nm) in a spectrophotometer.

• Excellent absorbance/emmitance results given by the blue and red filters near 580nm and 490nm respectively. 580nm is the wavelength where intensity of light is to be measured to identify the presence of mOrange. 490nm is the wavelength where maximum light intensity is expected with or without the presence of mOrange.